iFluor™ 488 Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH Alpaca Monoclonal Antibody (HA1129)
Catalog# HA1129
Alpaca Monoclonal to Alpaca anti-Rabbit IgG FC, Recombinant VHH
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IF-Cell
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IHC-Fr
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Rabbit
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (HA721174) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Alpaca anti-Rabbit IgG FC (iFluor™ 488, HA1129) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Specifications
Product Type
Alpaca monoclonal secondary
Product Name
iFluor™ 488 Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH Alpaca Monoclonal Antibody (HA1129)
Immunogen
Fc region of rabbit igg.
Host
Alpaca
Conjugation
Ifluor™ 488
Clonality
Monoclonal
APPLICATION DILUTION
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IF-Cell
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1:500-1:1,000
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IHC-Fr
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
Alpaca anti-Rabbit IgG FC, Recombinant VHH
FUNCTION
Higher resolution imaging due to the Alpaca anti-rabbit IgG Fc, recombinant VHHs’ 10 times smaller size than conventional secondary antibodies. The small size enables better tissue penetration, antigen access and decreases the distance between the epitope and label. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups.
CITATIONS
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Liu Guangzheng,et al
PGC-mediated conservation strategies for germplasm resources of Rugao Yellow Chicken and Shouguang Chicken in China
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IF
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Citation
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