IFNGR1 Recombinant Rabbit Monoclonal Antibody [JE40-50] (HA721952)
Catalog# HA721952
Rabbit Monoclonal to IFNGR1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (HA721952) at 1/1,000 dilution.
Lane 1: NCI-H226 cell lysate (20 µg/Lane)
Lane 2: LoVo cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: THP-1 cell lysate (20 µg/Lane)
Lane 5: K-562 cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: MCF7 cell lysate (negative) (20 µg/Lane)
Lane 8: HL-60 cell lysate (20 µg/Lane)
Lane 9: SW620 cell lysate (20 µg/Lane)
Lane 10: Mouse liver tissue lysate (40 µg/Lane)
Lane 11: Mouse thymus tissue lysate (40 µg/Lane)
Predicted band size: 54 kDa
Observed band size: 40-100 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721952) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: NCI-H226 cell lysate (20 µg/Lane)
Lane 2: LoVo cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: THP-1 cell lysate (20 µg/Lane)
Lane 5: K-562 cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: MCF7 cell lysate (negative) (20 µg/Lane)
Lane 8: HL-60 cell lysate (20 µg/Lane)
Lane 9: SW620 cell lysate (20 µg/Lane)
Lane 10: Mouse liver tissue lysate (40 µg/Lane)
Lane 11: Mouse thymus tissue lysate (40 µg/Lane)
Predicted band size: 54 kDa
Observed band size: 40-100 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721952) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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