IGFBP7 Recombinant Rabbit Monoclonal Antibody [JE34-01] (HA721423)
Catalog# HA721423
Rabbit Monoclonal to IGFBP7
Tested for
-
WB
-
IHC-P
-
IF-Cell
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of IGFBP7 on different lysates with Rabbit anti-IGFBP7 antibody (HA721423) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: SK-OV-3 cell lysate (15 µg/Lane)
Lane 3: SW480 cell lysate (15 µg/Lane)
Lane 4: 293T cell lysate (15 µg/Lane)
Lane 5: MCF7 cell lysate (15 µg/Lane)
Lane 6: Mouse kidney tissue lysate (30 µg/Lane)
Lane 7: Rat kidney tissue lysate (30 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721423) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: SK-OV-3 cell lysate (15 µg/Lane)
Lane 3: SW480 cell lysate (15 µg/Lane)
Lane 4: 293T cell lysate (15 µg/Lane)
Lane 5: MCF7 cell lysate (15 µg/Lane)
Lane 6: Mouse kidney tissue lysate (30 µg/Lane)
Lane 7: Rat kidney tissue lysate (30 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721423) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.