iNOS Recombinant Rabbit Monoclonal Antibody [JE59-61] (HA721583)
Catalog# HA721583
Rabbit Monoclonal to iNOS
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WB
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IF-Cell
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Human
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Mouse
Lane 1: RAW264.7 whole cell lysate
Lane 2: RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721583) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
iNOS Recombinant Rabbit Monoclonal Antibody [JE59-61] (HA721583)
Immunogen
Recombinant protein within human inos aa 1-300 / 1,153.
Host
Rabbit
Positive Control
RAW264.7 treated with 1μg/mL LPS for 24 hours whole cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE59-61
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 131 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
iNOS
SYNONYMS
HEP-NOS antibody;Hepatocyte NOS antibody;HEPNOS antibody;inducible antibody;Inducible nitric oxide synthase antibody;Inducible NO synthase antibody;Inducible NOS antibody;iNOS antibody;MAC NOS antibody;Macrophage NOS antibody;Nitric oxide synthase 2 inducible antibody;Nitric oxide synthase 2 inducible macrophage antibody;nitric oxide synthase 2A (inducible, hepatocytes) antibody;Nitric oxide synthase antibody;Nitric oxide synthase inducible antibody;nitric oxide synthase, macrophage antibody;NOS 2 antibody;NOS antibody;Nos II antibody;NOS type II antibody;nos2 antibody;NOS2_HUMAN antibody;NOS2A antibody;NOS2A, Inducible, Hepatocyte antibody;Peptidyl-cysteine S-nitrosylase NOS2 antibody
SUBCELLULAR LOCATION
Cytoplasm, cytosol.
FUNCTION
Nitric oxide synthase, inducible is an enzyme which is encoded by the NOS2 gene in humans and mice. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in two transcript variants encoding different isoforms. Nitric oxide synthase is expressed in epithelial cells of the liver, lung and bone marrow. It is inducible by a combination of lipopolysaccharide and certain cytokines. Nitric oxide is a reactive free radical mediating in neurotransmission, antimicrobial and antitumoral activities. In mice, the function of Nos2 in immunity against a number of viruses, bacteria, fungi, and parasites has been well characterized, whereas in humans the role of NOS2 has remained elusive and controversial. Nos2 is important for protective immunity against CMV. Caveolin 1 has been shown to interact with Nitric oxide synthase 2A. and Rac2.
CITATIONS
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Qin Danlei,et al
Mussel-inspired immunomodulatory and osteoinductive dual-functional hydroxyapatite nanoplatform for promoting bone regeneration
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WB
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Mouse
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Citation
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