IRF1 Recombinant Rabbit Monoclonal Antibody [SR44-08] (ET1602-28)
Catalog# ET1602-28
Rabbit Monoclonal to IRF1
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
IP
-
FC
-
Human
-
Mouse
-
Rat
Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: PC-12 cell lysate (20 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
IRF1 Recombinant Rabbit Monoclonal Antibody [SR44-08] (ET1602-28)
Immunogen
Recombinant protein within human irf1 aa 86-325 / 325.
Host
Rabbit
Positive Control
PC-12 cell lysate, Jurkat cell lysate, Hela, NIH/3T3, human colon carcinoma tissue, human breast carcinoma tissue, mouse brain tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SR44-08
RRID
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
IF-Cell
-
1:50
-
IF-Tissue
-
1:50
-
IHC-P
-
1:50-1:200
-
FC
-
1:1,000
-
IP
-
Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 37 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
IRF1
SYNONYMS
Interferon regulatory factor 1 antibody;Interferon regulatory factor 1 isoform +I9 antibody;Interferon regulatory factor 1 isoform d78 antibody;Interferon regulatory factor 1 isoform delta4 antibody;Interferon regulatory factor 1 isoform delta7 antibody;IRF 1 antibody;IRF-1 antibody;IRF1 antibody;IRF1_HUMAN antibody;MAR antibody;MAR1 antibody
SEQUENCE SIMILARITIES
Belongs to the IRF family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by CK2 and this positively regulates its activity.; Sumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-275. Sumoylation is enhanced by PIAS3 (By similarity). Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites.; Ubiquitinated. Appears to compete with sumoylation on C-terminal sites.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon- and ∫) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon- and interferon-∫ genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3 p48 (a component of the ISGF-3 complex) and IFN consensus sequence-binding protein (ICSBP).
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.