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Ki67 Recombinant Rabbit Monoclonal Antibody [SR00-02] (HA721115)

Price: Regular price $385.00

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Catalog# HA721115

Rabbit Monoclonal to Ki67

Tested for

  • IHC-P

  • IF-Tissue

  • mIHC

  • IF-Cell

  • FC

  • IHC-Fr

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Ki67 Recombinant Rabbit Monoclonal Antibody [SR00-02] (HA721115)

Immunogen

Synthetic peptide within human ki67 aa 1,040-1,080.

Host

Rabbit

Positive Control

Human tonsil tissue, human lymph nodes tissue, human cervical carcinoma tissue, human colon carcinoma tissue, mouse spleen tissue, mouse intestinal tissue, mouse ovary tissue, rat thymus tissue, human gastric cancer, HeLa.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR00-02

RRID

AB_3072239

APPLICATION DILUTION

  • IHC-P

  • 1:5,000-1:15,000

  • IF-Tissue

  • 1:500

  • mIHC

  • 1:2,000-1:3,000

  • IF-Cell

  • 1:100-1:250

  • FC

  • 1:500-1:1,000

  • IHC-Fr

  • 1:200

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 345-395 kDa

Isotype

IgG

TARGET

UNIPROT #

P46013

PROTEIN NAME

Ki67

SYNONYMS

Antigen identified by monoclonal antibody Ki 67 antibody;Antigen identified by monoclonal antibody Ki-67 antibody;Antigen KI-67 antibody;Antigen KI67 antibody;Antigen Ki67 antibody;KI67_HUMAN antibody;KIA antibody;Marker of proliferation Ki-67 antibody;MIB 1 antibody;MIB antibody;MKI67 antibody;PPP1R105 antibody;Proliferation marker protein Ki-67 antibody;Proliferation related Ki 67 antigen antibody;Protein phosphatase 1 regulatory subunit 105 antibody;RP11-380J17.2 antibody

SUBCELLULAR LOCATION

Nucleus, Chromosome.

FUNCTION

The Ki-67 protein is a nuclear protein doublet, 345-395 kDa, playing a pivotal role in maintaining cell proliferation. Ki-67 is present in all non-G0 phases of the cell cycle. Beginning in the mid G1, the level increases through S and G2 to reach a peak in M. In the end of M, is is rapidly catabolized. The Ki-67 labelling index (LI), i.e., the percentage of cells in a tissue staining for Ki-67, indicates the growth fraction. For many tumours, the rate of cell proliferation as assessed by Ki-67 immunoreactivity correlates with tumour grade and clinical course. In Non-Hodgkin lymphoma a labelling index of less than 20% is seen in low grade lymphomas, greater than 20% is associated with high grade lymphomas. Low grade lymphomas with a labelling index in excess of 5% have a worse prognosis than those with an index of less than 5%. In Burkitt and Burkitt-like lymphoma, nearly 100% of the nuclei are stained. This can be used as a diagnostic criterion. In gliomas the indices ranges from 0% to 5% for low grade astrocytomas while anaplastic astrocytomas and glioblastomas most frequently show an index above 10%. In soft tissue sarcomas Ki-67 index is positively correlated with mitotic count, cellularity and histological grade. In some benign tumours, like meningioma, a high LI is associated with a high recurrence rate. In dysplasia in Barrett's oesophagus and in granulosa cell tumours and ovarian serous tumours, Ki-67 LI is associated with progression. In the former, reproducibility of dysplasia grading is improved when Ki67 is included. In breast cancer, the proliferative index measured by Ki67 immunoreactivity has both prognostic and predictive value.

CITATIONS

  • Zhang Kangnan,et al

    Disulfidptosis-related gene expression reflects the prognosis of drug-resistant cancer patients and inhibition of MYH9 reverses sorafenib resistance

  • Yifeng Wei ,et al

    Nicotine aggravates high-fat diet-induced non-alcoholic fatty liver disease in mice via inhibition of CISD3

  • Wang Pengcheng,et al

    Liposome Nanomedicine Based on Tumor Cell Lysate Mitigates the Progression of Lynch Syndrome-Associated Colon Cancer

  • Zhu Zhaoying,et al

    IKIP downregulates THBS1/FAK signaling to suppress migration and invasion by glioblastoma cells

  • Value of whole-body dynamic 18F-FMISO PET/CT Patlak multi-parameter imaging for evaluating the early radiosensitizing effect of oleanolic acid on C6 rat gliomas

  • Dai Chao,et al

    Identifying SLC2A6 as the novel protective factor in breast cancer by TP53-related genes affecting M1 macrophage infiltration

  • Wang M, Li Y, Yang X, et al

    Effects of metronidazole on colorectal cancer occurrence and colorectal cancer liver metastases by regulating Fusobacterium nucleatum in mice

  • Peng Zhiwei,et al

    miR-135b-5p promotes gastric carcinogenesis by targeting CLIP4-mediated JAK2/STAT3 signal pathway

  • Peng Zhiwei,et al

    Crosstalk between cancer-associated fibroblasts and myeloid cells shape the heterogeneous microenvironment of gastric cancer

  • Congcong Lu,et al

    Shikonin induces ferroptosis in osteosarcomas through the mitochondrial ROS-regulated HIF-1α/HO-1 axis

  • Wu Y, Chen L, Feng C, et al

    Antitumor effect of toosendanin on oral squamous cell carcinoma via suppression of p-STAT3

  • Xia, L., Liu, X., Mao, W., Guo, Y., Huang, J., Hu, Y., Jin, L., Liu, X., Fu, H., Du, Y., & Shou, Q.

    Panax notoginseng saponins normalises tumour blood vessels by inhibiting EphA2 gene expression to modulate the tumour microenvironment of breast cancer

  • Jiang Lanxin,et al

    Nociceptive adenosine A2A receptor on trigeminal nerves orchestrates CGRP release to regulate the progression of oral squamous cell carcinoma

  • Wu Ziying,et al

    Multimodal enhancement of ferroptosis for synergistic cascade colorectal cancer therapy

  • AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression

  • Gao Yangshuai,et al

    Nr4a1 enhances Wnt4 transcription to promote mesenchymal stem cell osteogenesis and alleviates inflammation-inhibited bone regeneration

  • Li, C., Qin, T., Jin, Y., Hu, J., Yuan, F., Cao, Y., & Duan, C.

    Cerebrospinal fluid-derived extracellular vesicles after spinal cord injury promote vascular regeneration via PI3K/AKT signaling pathway

  • Liu Junhao,et al

    Tanshinol ameliorates imiquimod-induced psoriasis by inhibiting M1 macrophage polarization through suppression of the notch signaling pathway

  • CDC42‑mediated Wnt signaling facilitates odontogenic differentiation of DPCs during tooth root elongation

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