LAMP1 Recombinant Rabbit Monoclonal Antibody [JJ0940] (ET1701-94)
Catalog# ET1701-94
Rabbit Monoclonal to LAMP1
-
WB
-
IHC-P
-
IF-Tissue
-
Human
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HUVEC cell lysate (20 µg/Lane)
Lane 5: Human kidney tissue lysate (40 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 100-120 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-94) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
LAMP1 Recombinant Rabbit Monoclonal Antibody [JJ0940] (ET1701-94)
Immunogen
Recombinant protein within human lamp1 aa 84-206 / 417.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, HUVEC cell lysate, human kidney tissue lysate, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ0940
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
IHC-P
-
1:5,000
-
IF-Tissue
-
1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 45 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
LAMP1
SYNONYMS
CD107 antigen like family member A antibody;CD107 antigen-like family member A antibody;CD107a antibody;CD107a antigen antibody;LAMP 1 antibody;LAMP-1 antibody;LAMP1 antibody;LAMP1_HUMAN antibody;LAMPA antibody;LGP120 antibody;lgpA antibody;Lysosomal membrane glycoprotein 120KD antibody;Lysosomal Associated Membrane Protein 1 antibody;Lysosome associated membrane glycoprotein 1 antibody;Lysosome-associated membrane glycoprotein 1 antibody;Lysosome-associated membrane protein 1 antibody;OTTHUMP00000040663 antibody
SEQUENCE SIMILARITIES
Belongs to the LAMP family.
POST-TRANSLATIONAL MODIFICATION
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.
SUBCELLULAR LOCATION
Cell membrane, Endosome membrane, Lysosome membrane, Late endosome.
FUNCTION
Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene. The human LAMP1 gene is located on the long arm (q) of chromosome 13 at region 3, band 4 (13q34).Immunofluorescence staining of HeLa Cells with antibody to reveal lysosomal LAMP1 in red and vimentin containing intermediate filaments in green. Nuclear DNA is seen in blue. Antibodies and image courtesy EnCor Biotechnology Inc.Lysosomal-associated membrane protein 1 is a glycoprotein from a family of Lysosome-associated membrane glycoproteins.The LAMP-1 glycoprotein is a type I transmembrane protein which is expressed at high or medium levels in at least 76 different normal tissue cell types.It resides primarily across lysosomal membranes,and functions to provide selectins with carbohydrate ligands. CD107a has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells. and may also play a role in tumor cell differentiation and metastasis.
CITATIONS
-
Ping Yang
Cellular Evidence and Source of Exosomes in the Biliary System of the Chinese Soft-Shelled Turtle,Pelodiscus sinensis
-
WB
-
IHC
-
Pelodiscus sinensis
-
Citation
-
