LAP3 Recombinant Rabbit Monoclonal Antibody [PSH01-26] (HA721650)
Catalog# HA721650
Rabbit Monoclonal to LAP3
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of LAP3 on different lysates with Rabbit anti-LAP3 antibody (HA721650) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HepG2 cell lysate (15 µg/Lane)
Lane 3: U-87 MG cell lysate (15 µg/Lane)
Lane 4: Daudi cell lysate (15 µg/Lane)
Lane 5: C6 cell lysate (15 µg/Lane)
Lane 6: Mouse liver tissue lysate (30 µg/Lane)
Lane 7: Rat liver tissue lysate (30 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 50 kDa
Exposure time: Lane 1-7 (left): 1 minute; Lane 1-7 (right): 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721650) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HepG2 cell lysate (15 µg/Lane)
Lane 3: U-87 MG cell lysate (15 µg/Lane)
Lane 4: Daudi cell lysate (15 µg/Lane)
Lane 5: C6 cell lysate (15 µg/Lane)
Lane 6: Mouse liver tissue lysate (30 µg/Lane)
Lane 7: Rat liver tissue lysate (30 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 50 kDa
Exposure time: Lane 1-7 (left): 1 minute; Lane 1-7 (right): 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721650) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.