Ly6c Rabbit Polyclonal Antibody (HA500087)
Catalog# HA500087
Rabbit Polyclonal to Ly6c
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WB
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IHC-P
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Mouse
Lane 1: C2C12 cell lysate
Lane 2: Mouse lung tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: Mouse kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 1 minute 50 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500087) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Ly6c Rabbit Polyclonal Antibody (HA500087)
Immunogen
Synthetic peptide within mouse ly6c2 aa 45-77.
Host
Rabbit
Positive Control
C2C12 cell lysate, Mouse lung tissue lysate, Mouse spleen tissue lysate, Mouse kidney tissue lysate, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
-
WB
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1:2,000
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 14 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Ly6c
SYNONYMS
Ly 6c antibody;Ly6c protein antibody;Ly6c1 antibody;Ly6c2 antibody;Lymphocyte antigen 6 complex antibody;Lymphocyte antigen 6 complex locus C antibody;Lymphocyte antigen 6C antibody;Lymphocyte antigen 6C1 antibody;Lymphocyte antigen 6C2 antibody;Lymphocyte antigen Ly 6C antibody
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
The gene encoding the mouse alloantigen, Ly-6C, maps to chromosome 15 and encodes a 131 amino acid protein that belongs to the Ly-6 family of glycosyl-phosphatidylinositol (GPI)-linked proteins. Ly-6 family members share amino acid homology throughout a distinctive cysteine rich protein domain that incorporates O-linked carbohydrates. Murine Ly-6 molecules have unique patterns of tissue expression during hematopoiesis from multipotential stem cells to lineage committed precursor cells, and on specific leukocyte subpopulations in the peripheral lymphoid tissues. Ly-6C is predominantly expressed on murine peripheral CD8 T cells. Ly-6C is involved in endothelial adhesion, the killing of target cells by CTLs, inducing TCR-mediated activation of IL-2 and IFN-γ production in CD8 T cells and the homing of CD8 T cells. In addition, Ly-6C may act as a signaling molecule of LFA-1 activation.
CITATIONS
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Integrated Bioinformatics Analysis and Experimental Verification of Immune Cell Infiltration and the Related Core Genes in Ulcerative Colitis
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IHC
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Mouse
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Citation
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