Monoamine Oxidase A / MAO-A Mouse Monoclonal Antibody [5D1] (EM1701-54)
Catalog# EM1701-54
Mouse Monoclonal to MAO-A
Tested for
-
WB
-
IF-Cell
Reacts with
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Human
-
Mouse
-
Rat
Western blot analysis of MAO-A on different lysates with Mouse anti-MAO-A antibody (EM1701-54) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: SiHa cell lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 70 kDa
Exposure time: Lane 1-2: 46 seconds; Lane 3-4: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: SiHa cell lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 70 kDa
Exposure time: Lane 1-2: 46 seconds; Lane 3-4: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.