mCherry Mouse Monoclonal Antibody [A9F2] (HA601186)
Catalog# HA601186
Mouse Monoclonal to mCherry
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WB
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IF-Cell
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Species independent
Western blot analysis of mCherry on different lysates with Mouse anti-mCherry antibody (HA601186) at 1/2,000 dilution.
Lane 1: 293T-WT cell lysate (5 µg/Lane)
Lane 2: 293T-OE-mCherry cell lysate (5 µg/Lane)
Lane 3: 293T-OE-mCherry cell lysate (2.5 µg/Lane)
Lane 4: 293T-OE-mCherry cell lysate (1.25 µg/Lane)
Lane 5: 293T-OE-mCherry cell lysate (0.625 µg/Lane)
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601186) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/150,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
mCherry Mouse Monoclonal Antibody [A9F2] (HA601186)
Immunogen
Recombinant protein within mcherry aa 1-236 / 236.
Host
Mouse
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A9F2
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:5,000
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IF-Cell
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1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1.17ug/ul
Purification
Protein A affinity purified.
Isotype
IgG2b
TARGET
UNIPROT #
PROTEIN NAME
mCherry
FUNCTION
mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As a RFP, mCherry was derived from DsRed of Discosoma sea anemones unlike green fluorescent proteins (GFPs) which are often derived from Aequoera victoria jellyfish.Fluorescent proteins are used to tag components in the cell, so they can be studied using fluorescence spectroscopy and fluorescence microscopy. mCherry absorbs light between 540-590 nm and emits light in the range of 550-650 nm. mCherry belongs to the group of fluorescent protein chromophores used as instruments to visualize genes and analyze their functions in experiments. Genome editing has been improved greatly through the precise insertion of these fluorescent protein tags into the genetic material of many diverse organisms. Most comparisons between the brightness and photostability of different fluorescent proteins have been made in vitro, removed from biological variables that affect protein performance in cells or organisms. It is hard to perfectly simulate cellular environments in vitro, and the difference in environment could have an effect on the brightness and photostability.
CITATIONS
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Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus
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Co-IP
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Human
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Citation
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