MCM2 Recombinant Rabbit Monoclonal Antibody [JE42-39] (HA721553)
Catalog# HA721553
Rabbit Monoclonal to MCM2
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: HL-60 cell lysate (20 µg/Lane)
Lane 6: Mouse thymus tissue lysate (40 µg/Lane)
Lane 7: Jurkat cell lysate (20 µg/Lane)
Lane 8: C2C12 cell lysate (20 µg/Lane)
Lane 9: U-87 MG cell lysate (20 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 130 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721553) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
MCM2 Recombinant Rabbit Monoclonal Antibody [JE42-39] (HA721553)
Immunogen
Synthetic peptide within human mcm2 aa 31-80 / 904.
Host
Rabbit
Positive Control
SW480 cell lysate, SH-SY5Y cell lysate, Raji cell lysate, HeLa cell lysate, HL-60 cell lysate, mouse thymus tissue lysate, Jurkat cell lysate, C2C12 cell lysate, U-87 MG cell lysate, human lymph nodes tissue, human small intestine tissue, human testis tissue, HeLa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE42-39
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Cell
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1:250
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 102 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MCM2
SYNONYMS
BM28 antibody;CCNL 1 antibody;CCNL1 antibody;CDC like 1 antibody;cdc19 antibody;CDCL 1 antibody;CDCL1 antibody;Cell devision cycle like 1 antibody;Cyclin like 1 antibody;D3S3194 antibody;DNA replication licensing factor MCM2 antibody;KIAA0030 antibody;MCM 2 antibody;MCM2 antibody;MCM2 minichromosome maintenance deficient 2 mitotin antibody;MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody;MCM2 minichromosome maintenance deficient 2, mitotin antibody;MCM2_HUMAN antibody;MGC10606 antibody;Minichromosome maintenance complex component 2 antibody;Minichromosome maintenance deficient 2 (mitotin) antibody;Minichromosome maintenance deficient 2 mitotin antibody;Minichromosome maintenance protein 2 antibody;Minichromosome maintenance protein 2 homolog antibody;Mitotin antibody;Nuclear protein BM28 antibody;OTTHUMP00000216047 antibody;OTTHUMP00000216050 antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
DNA replication licensing factor MCM2 is a protein that in humans is encoded by the MCM2 gene. The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication-related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
CITATIONS
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Dongchao Xu,et al
Isolinderalactone suppresses pancreatic ductal adenocarcinoma by activating p38 MAPK to promote DDIT3 expression and trigger endoplasmic reticulum stress
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WB
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Human
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Citation
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