MCM7 Mouse Monoclonal Antibody [15E1] (EM1901-12)
Catalog# EM1901-12
Mouse Monoclonal to MCM7
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
-
Monkey
Western blot analysis of MCM7 on different lysates with Mouse anti-MCM7 antibody (EM1901-12) at 1/1,000 dilution.
Lane 1: HL-60 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lane 4: Jurkat cell lysate
Lane 5: SW480 cell lysate
Lane 6: LNCaP cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: C2C12 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 81 kDa
Observed band size: 81 kDa
Exposure time: 51 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HL-60 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lane 4: Jurkat cell lysate
Lane 5: SW480 cell lysate
Lane 6: LNCaP cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: C2C12 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 81 kDa
Observed band size: 81 kDa
Exposure time: 51 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.