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MDM2 Recombinant Mouse Monoclonal Antibody [A10E11-R] (HA601310)

Price: Regular price $350.00

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Catalog# HA601310

Mouse Monoclonal to MDM2

Tested for

  • WB

  • IF-Cell

  • IHC-P

Reacts with

  • Human

  • Monkey

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free

Specifications

Product Type

Recombinant Mouse monoclonal primary

Product Name

MDM2 Recombinant Mouse Monoclonal Antibody [A10E11-R] (HA601310)

Immunogen

Recombinant protein within human mdm2 aa 1-491 / 491.

Host

Mouse

Positive Control

U-2 OS treated with 10μM NUTLIN 3A for 24 hours cell lysate, HepG2 cell lysate, COS-1cell lysate, U-2 OS cells treated with 10μM NUTLIN 3A for 24 hours, human embryonal carcinoma tissue, human testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A10E11-R

RRID

AB_3096634

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IF-Cell

  • 1:500

  • IHC-P

  • 1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 55 kDa

Isotype

IgG1

TARGET

UNIPROT #

Q00987

PROTEIN NAME

MDM2

SYNONYMS

ACTFS antibody;Double minute 2 protein antibody;E3 ubiquitin-protein ligase Mdm2 antibody;Hdm 2 antibody;Hdm2 antibody;HDMX antibody;MDM 2 antibody;MDM2 antibody;MDM2 oncogene E3 ubiquitin protein ligase antibody;Mdm2 p53 E3 ubiquitin protein ligase homolog antibody;Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody;MDM2_HUMAN antibody;MDM2BP antibody;Mouse Double Minute 2 antibody;MTBP antibody;Murine Double Minute Chromosome 2 antibody;Oncoprotein Mdm2 antibody;p53 Binding Protein Mdm2 antibody;p53-binding protein Mdm2 antibody;Ubiquitin protein ligase E3 Mdm2 antibody;Ubiquitin protein ligase E3 Mdm2 antibody

SUBCELLULAR LOCATION

Nucleus, nucleoplasm, nucleolus, Cytoplasm.

FUNCTION

Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by the MDM2 gene. Mdm2 is an important negative regulator of the p53 tumor suppressor. Mdm2 protein functions both as an E3 ubiquitin ligase that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and as an inhibitor of p53 transcriptional activation.

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