MGMT Rabbit Polyclonal Antibody (ER40104)
Catalog# ER40104
Rabbit Polyclonal to MGMT
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
Positive control:
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Daudi cell lysate
Lane 4: Raji cell lysate
Lane 5: SK-BR-3 cell lysate
Lane 6: SKOV3 cell lysate
Lane 7: Human liver tissue lysate
Lane 8: Human kidney tissue lysate
Lane 9: Human thymus tissue lysate
Lane 10: Human placentae tissue lysate
Specifications
Product Type
Rabbit polyclonal primary
Product Name
MGMT Rabbit Polyclonal Antibody (ER40104)
Immunogen
Synthetic peptide within residues of mgmt aa 158-207 / 207.
Host
Rabbit
Positive Control
MCF-7 cell lysate, Hela cell lysate, Dauai cell lysate, Raji cell lysate, SK-BR-3 cell lysate, SKOV3 cell lysate, human kidney tissue lysate, human liver tissue lysate, human thymus tissue lysate, human placentae tissue lysate, A172, SK-BR-3, MCF-7, human tonsil tissue, human colon carcinoma tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
IF-Cell
-
1:200
-
IHC-P
-
1:200
-
FC:1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 22 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MGMT
SYNONYMS
6 O methylguanine DNA methyltransferase antibody;6-O-methylguanine-DNA methyltransferase antibody;Agat antibody;AGT antibody;AI267024 antibody;EC 2.1.1.63 antibody;Methylated DNA protein cysteine methyltransferase antibody;Methylated-DNA--protein-cysteine methyltransferase antibody;Methylguanine DNA methyltransferase antibody;MGC107020 antibody;MGMT antibody;MGMT_HUMAN antibody;O 6 methylguanine DNA alkyltransferase antibody;O 6 methylguanine DNA methyltransferase antibody;O-6-methylguanine-DNA methyltransferase antibody;O-6-methylguanine-DNA-alkyltransferase antibody
SEQUENCE SIMILARITIES
Belongs to the MGMT family.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O (6) position of guanine, restoring guanine to its normal form without causing DNA breaks. MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents. It is ubiquitously expressed in normal human tissues and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy.
CITATIONS
-
Pei, J., Su, Z., Zeng, X., Zhong, Y., Zhang, Y., Yang, Y., Lu, Q., Li, J., & Deng, Y.
Protocatechuic aldehyde acts synergistically with dacarbazine to augment DNA double-strand breaks and promote apoptosis in cutaneous melanoma cells
-
WB
-
Citation
-
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.