MLH1 Mouse Monoclonal Antibody [A9F7] (HA601135)
Catalog# HA601135
Mouse Monoclonal to MLH1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Knock-Out or Knock-Down validated
Western blot analysis of MLH1 on different lysates with Mouse anti-MLH1 antibody (HA601135) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HCT 116 cell lysate (negative) (15 µg/Lane)
Lane 3: A549 cell lysate (15 µg/Lane)
Lane 4: HepG2 cell lysate (15 µg/Lane)
Lane 5: Jurkat cell lysate (15 µg/Lane)
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: Lane 1-5 (left): 24 seconds; Lane 1-5 (right): 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601135) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HCT 116 cell lysate (negative) (15 µg/Lane)
Lane 3: A549 cell lysate (15 µg/Lane)
Lane 4: HepG2 cell lysate (15 µg/Lane)
Lane 5: Jurkat cell lysate (15 µg/Lane)
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: Lane 1-5 (left): 24 seconds; Lane 1-5 (right): 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601135) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
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