MMP-11 Recombinant Rabbit Monoclonal Antibody [SN74-08] (ET1611-33)
Catalog# ET1611-33
Rabbit Monoclonal to MMP-11
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
Lane 1: 293T cell lysate (15 µg/Lane)
Lane 2: MCF7 cell lysate (15 µg/Lane)
Lane 3: Mouse kidney tissue lysate (20 µg/Lane)
Lane 4: Mouse lung tissue lysate (20 µg/Lane)
Lane 5: Mouse testis tissue lysate (20 µg/Lane)
Lane 6: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-33) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
MMP-11 Recombinant Rabbit Monoclonal Antibody [SN74-08] (ET1611-33)
Immunogen
Synthetic peptide within human mmp11 aa 439-488 / 488.
Host
Rabbit
Positive Control
293T cell lysate, MCF7 cell lysate, mouse kidney tissue lysate, mouse lung tissue lysate, mouse testis tissue lysate, rat testis tissue lysate, SW480, MCF-7, A549, human spleen tissue, human breast carcinoma tissue, mouse spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN74-08
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:500-1:1,000
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IF-Tissue
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1:500-1:1,000
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IHC-P
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1:100-1:5000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 55 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MMP-11
SYNONYMS
Matrix Metalloproteinase 11 antibody;Matrix metalloproteinase-11 antibody;MMP-11 antibody;Mmp11 antibody;MMP11_HUMAN antibody;SL 3 antibody;SL-3 antibody;SL3 antibody;ST3 antibody;STMY3 antibody;Stromelysin 3 antibody;Stromelysin III antibody;Stromelysin-3 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase M10A family.
TISSUE SPECIFICITY
Specifically expressed in stromal cells of breast carcinomas.
POST-TRANSLATIONAL MODIFICATION
The precursor is cleaved by a furin endopeptidase.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including Collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also called stromelysin-1, -2 and -3) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies.
CITATIONS
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Xu, J., Fang, Y., Chen, K., Li, S., Tang, S., Ren, Y., Cen, Y., Fei, W., Zhang, B., Shen, Y., & Lu, W.
Single-Cell RNA Sequencing Reveals the Tissue Architecture in Human High-Grade Serous Ovarian Cancer
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IF
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Human
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Citation
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