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HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76] (M1005-3)

Price: Regular price $374.00

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Catalog# M1005-3

Mouse Monoclonal to Mouse anti-Human IgG heavy chain

Tested for

  • WB

  • ELISA

Reacts with

  • Human

Conjugated to HRP

Specifications

Product Type

Mouse monoclonal primary

Product Name

HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76] (M1005-3)

Immunogen

Purified human igg heavy chain.

Host

Mouse

Positive Control

Human serum lysates.

Conjugation

Hrp

Clonality

Monoclonal

Clone Number

54-76

RRID

AB_3073015

APPLICATION DILUTION

  • WB

  • 1:5,000

  • ELISA

  • 1:5,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.

Concentration

2ug/ul

Purification

Immunogen affinity purified.

Molecular Weight

Predicted band size: 50 kDa

Isotype

IgG2a

TARGET

PROTEIN NAME

Mouse anti-Human IgG heavy chain

SUBCELLULAR LOCATION

serum plasma

FUNCTION

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

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