MRP1 Recombinant Rabbit Monoclonal Antibody [JA93-03] (ET1704-56)
Catalog# ET1704-56
Rabbit Monoclonal to MRP1
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WB
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IHC-P
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FC
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IF-Cell
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Human
Lane 1: A549 cell lysate
Lane 2: A549 cell lysate (no heat)
Lane 3: HepG2 cell lysate
Lane 4: HepG2 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 172 kDa
Observed band size: 220 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-56) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
MRP1 Recombinant Rabbit Monoclonal Antibody [JA93-03] (ET1704-56)
Immunogen
Synthetic peptide within human mrp1 aa 1482-1531 / 1531.
Host
Rabbit
Positive Control
A549 cell lysate, HepG2 cell lysate, A549, human pancreas tissue, human tonsil tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA93-03
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:50-1:200
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FC
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 172 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MRP1
SYNONYMS
ABC 29 antibody;ABC29 antibody;ABCC 1 antibody;ABCC antibody;Abcc1 antibody;ATP binding cassette sub family C (CFTR/MRP) member 1 antibody;ATP binding cassette sub-family C member 1 antibody;ATP binding cassette subfamily C member 1 antibody;ATP binding cassette transporter variant ABCC1delta ex13 antibody;ATP binding cassette transporter variant ABCC1delta ex13&14 antibody;ATP binding cassette transporter variant ABCC1delta ex25 antibody;ATP binding cassette transporter variant ABCC1delta ex25&26 antibody;ATP binding cassette, sub-family C (CFTR/MRP), member 1 antibody;ATP-binding cassette sub-family C member 1 antibody;DKFZp686N04233 antibody;DKFZp781G125 antibody;GS X antibody;GSX antibody;Leukotriene C(4) transporter antibody;LTC4 transporter antibody;MRP 1 antibody;MRP antibody;MRP1 antibody;MRP1_HUMAN antibody;Multidrug resistance associated protein 1 antibody;Multidrug resistance protein antibody;Multidrug resistance-associated protein 1 antibody;Multiple drug resistance associated protein antibody;Multiple drug resistance protein 1 antibody
SEQUENCE SIMILARITIES
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
TISSUE SPECIFICITY
Lung, testis and peripheral blood mononuclear cells.
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the Mdr-1 P-glycoprotein and the multidrug-resistance protein MRP1. MRP1 is an integral membrane protein that contains an Mdr-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood. The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter) and MRP3, which are both conjugate export pumps expressed predominantly in hepatocytes. MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells. Conversely, MRP3 localizes to the basolateral membrane where it also mediates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane. MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired. MRP6 protein is highly expressed in liver and kidney, whereas MRP4 and MRP5 are detected in various tissues yet at much lower levels of expression.
CITATIONS
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Doxorubicin resistance in breast cancer is mediated via the activation of FABP5/PPARγ and CaMKII signaling pathway
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WB
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Human
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Citation
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