MSH6 Recombinant Rabbit Monoclonal Antibody [SP08-02] (ET1604-39)
Catalog# ET1604-39
Rabbit Monoclonal to MSH6
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
Lysates/proteins at 20 µg/Lane.
Predicted band size: 153 kDa
Observed band size: 153 kDa
Exposure time: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-39) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
MSH6 Recombinant Rabbit Monoclonal Antibody [SP08-02] (ET1604-39)
Immunogen
Synthetic peptide within human msh6 aa 1-50 / 1,360.
Host
Rabbit
Positive Control
A549 cell lysates, A549, RAW264.7, human tonsil tissue, mouse epididymis tissue, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SP08-02
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:100
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 153 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MSH6
SYNONYMS
DNA mismatch repair protein Msh6 antibody;G/T mismatch binding protein antibody;G/T mismatch-binding protein antibody;GTBP antibody;GTMBP antibody;hMSH6 antibody;HNPCC 5 antibody;HNPCC5 antibody;HSAP antibody;MSH 6 antibody;MSH6 antibody;MSH6_HUMAN antibody;mutS (E. coli) homolog 6 antibody;MutS alpha 160 kDa subunit antibody;MutS homolog 6 (E. coli) antibody;mutS homolog 6 antibody;MutS-alpha 160 kDa subunit antibody;p160 antibody;Sperm associated protein antibody
SEQUENCE SIMILARITIES
Belongs to the DNA mismatch repair MutS family.
POST-TRANSLATIONAL MODIFICATION
The N-terminus is blocked.; Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
SUBCELLULAR LOCATION
Nucleus, Chromosome.
FUNCTION
Multiple pathways promote short-sequence recombination (SSR) in Saccharomyces cerevisiae. When gene conversion is initiated by a double-strand break (DSB), any nonhomologous DNA that may be present at the ends must be removed before new DNA synthesis can be initiated. Removal of a 3' nonhomologous tail in S. cerevisiae depends on the nucleotide excision repair endonuclease Rad1/Rad10 and also on the mismatch repair proteins Msh2 and Msh3. Msh2 and Msh3, which function in mitotic recombination, recognize not only heteroduplex loops and mismatched basepairs, but also branched DNA structures with a free 3' tail. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication. Yeast Msh2-Msh6 interact asymmetrically with the DNA through base-specific stacking and hydrogen bonding interactions and backbone contacts. The importance of these contacts decreases with increasing distance from the mismatch, implying that interactions at or near the mismatch are important for binding in a kinked DNA conformation.
CITATIONS
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Li Y, Zhang Y, Shah SB, Chang CY, Wang H, Wu X
MutSβ protects common fragile sites by facilitating homology-directed repair at DNA double-strand breaks with secondary structures
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WB
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Human
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Citation
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