Myc tag Rabbit Polyclonal Antibody (R1208-1)
Catalog# R1208-1
Rabbit Polyclonal to Myc tag
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WB
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IP
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IF-Cell
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FC
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Species independent
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Myc tag Rabbit Polyclonal Antibody (R1208-1)
Immunogen
Synthetic peptide within human myc aa 410-420.
Host
Rabbit
Positive Control
PG-CM cell lysates, C-terminal Myc-tagged recombinant protein, N-terminal Myc-tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IP
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2-5 µg/ml.
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IF-Cell
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1:200
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Myc tag
SYNONYMS
avian myelocytomatosis viral oncogene homolog antibody;bHLHe39 antibody;c-Myc antibody;class E basic helix-loop-helix protein 39 antibody;MRTL antibody;MYC antibody;Myc Epitope Tag antibody;myc proto-oncogene protein antibody;myc-related translation/localization regulatory factor antibody;oncogene c-Myc antibody;proto-oncogene c-Myc antibody;protooncogene homologous to myelocytomatosis virus antibody;transcription factor p64 antibody;v-myc avian myelocytomatosis viral oncogene homolog antibody;v-myc myelocytomatosis viral oncogene homolog (avian) antibody
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.; Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).
FUNCTION
Myc gene encodes for a transcription factor that is believed to regulate expression of 15% of all genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. This Myc-Tag antibody detects Myc-tagged fusion proteins.
CITATIONS
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Shui Bo Xu,et al
KPNA3 regulates histone locus body formation by modulating condensation and nuclear import of NPAT
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WB
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Human
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Citation
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Jiyong Zhou
Ubiquitination Is Essential for Avibirnavirus Replication by Supporting VP1 Polymerase Activity
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WB
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human
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Citation
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Zhou, J., Wang, Y., Qiu, Y., Wang, Y., Yang, X., Liu, C., Shi, Y., Feng, X., Hou, L., & Liu, J.
Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein
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WB
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Pig
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Citation
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Cao, J., Liu, S., Liu, M., Wang, S., Bi, Z., Fan, W., Shi, Z., Song, S., & Yan, L.
Hsp70 Inhibits the Replication of Fowl Adenovirus Serotype 4 by Suppressing Viral Hexon with the Assistance of DnaJC7
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WB
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Chicken
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Citation
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Zheng Jiaqi,et al
An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination
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WB
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Human
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Citation
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Jiyong Zhou
The co-chaperone Cdc37 regulates the rabies virus phosphoprotein stability by targeting to Hsp90AA1 machinery
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WB
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mouse
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Citation
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Mu Mingshan,et al
FAM188B promotes the growth, metastasis, and invasion of hepatocellular carcinoma by targeting the HRNPA1/PKM2 axis
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WB
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Human
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Citation
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Deng, T., Hu, B., Wang, X., Yan, Y., Zhou, J., Lin, L., Xu, Y., Zheng, X., & Zhou, J.
DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication
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WB
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Human
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Citation
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Plaat1 promotes P53 degradation Via autophagy-lysosome pathway in zebrafish
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WB
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Human
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Citation
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p73 coordinates with Δ133p53 to promote DNA double-strand break repair
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IP
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Human
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Citation
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Convergent and divergent regulations of ethylene and abscisic acid biosynthesis during persimmon fruit postharvest ripening
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WB
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Co-IP
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Persimmon fruit
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Nicotiana benthamiana
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Citation
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Qin, Q., Shou, J., Li, M., Gu, M., Meng, Z., Xu, P., Meng, H., & Wang, X.
Stk24 protects against obesity-associated metabolic disorders by disrupting the NLRP3 inflammasome. Cell reports, 35(8), 109161.
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WB
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Citation
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Bi, Z., Wang, W., & Xia, X.
Structure and function of a novel lineage-specific neutralizing epitope on H protein of canine distemper virus
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Jiyong Zhou
Caspase-Dependent Apoptosis Induction via Viral Protein ORF4 of Porcine Circovirus 2 Binding to Mitochondrial Adenine Nucleotide Translocase 3
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WB
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Porcine Circovirus
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Citation
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Jiyong Zhou
Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins
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WB
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pig
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Citation
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Wu, H., Yang, H., Ji, G., Zheng, T., Zhang, Y., Deng, T., Zheng, X., Zhou, J., & Hu, B.
SUMO1 Modification Facilitates Avibirnavirus Replication by Stabilizing Polymerase VP1. Journal of virology, 93(10), e02227-18.
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WB
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Citation
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Deng T, Du L, Ding S, et al
Protein kinase Cdc7 supports viral replication by phosphorylating Avibirnavirus VP3 protein
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WB
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Human
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Citation
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Jinyan Gu; Jiyong Zhou
Nucleolar protein NPM1 is essential for circovirus replication by binding to viral capsid
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WB
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CoIP
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human
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Citation
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Jian Chen;Fengdong Zhao
The RCAN1.4-calcineurin/NFAT signaling pathway is essential for hypoxic adaption of intervertebral discs
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WB
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IP
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human
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Citation
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Jin Kai,et al
BZW2 promotes malignant progression in lung adenocarcinoma through enhancing the ubiquitination and degradation of GSK3β
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WB
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IP
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Human
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Citation
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Di Wang
Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation
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WB
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IP
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Mouse
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Citation
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Lin, C., Hu, J., Dai, Y., Zhang, H., Xu, K., Dong, W., Yan, Y., Peng, X., Zhou, J., & Gu, J.
Porcine Circovirus Type 2 Hijacks Host IPO5 to Sustain the Intracytoplasmic Stability of Its Capsid Protein
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Duan Chunyan,et al
E3 ubiquitin ligase UBR5 modulates circadian rhythm by facilitating the ubiquitination and degradation of the key clock transcription factor BMAL1
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WB
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IP
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Human
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Citation
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Xu, C., Li, T., Lei, J., Zhang, Y., Zhou, J., & Hu, B.
The Autophagy Cargo Receptor SQSTM1 Inhibits Infectious Bursal Disease Virus Infection through Selective Autophagic Degradation of Double-Stranded Viral RNA
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IF
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Citation
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Feng, Z., Tao, S., Huang, Z., Zheng, B., Kong, X., Xiang, Y., Zhang, Q., Song, H., Xu, Z., Wei, X., Zhao, F., & Chen, J.
The deubiquitinase UCHL1 negatively controls osteoclastogenesis by regulating TAZ/NFATC1 signalling
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WB
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Human
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Citation
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PLAAT1 inhibits type I interferon response via degradation of IRF3 and IRF7 in Zebrafish
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IB
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Human
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Citation
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Li, F. L., Liu, J. P., Bao, R. X., Yan, G., Feng, X., Xu, Y. P., Sun, Y. P., Yan, W., Ling, Z. Q., Xiong, Y., Guan, K. L., & Yuan, H. X.
Acetylation accumulates PFKFB3 in cytoplasm to promote glycolysis and protects cells from cisplatin-induced apoptosis
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IF
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Human
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Citation
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Mo, Y., Lin, R., Liu, P., Tan, M., Xiong, Y., Guan, K. L., & Yuan, H. X.
SIRT7 deacetylates DDB1 and suppresses the activity of the CRL4 E3 ligase complexes. The FEBS journal, 284(21), 3619–3636.
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WB
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Citation
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Boli Hu;Jiyong Zhou
Influenza A Virus Induces Autophagy by Its Hemagglutinin Binding to Cell Surface Heat Shock Protein 90AA1
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WB
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CoIP
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Chicken
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Citation
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Huang, Y., Duan, X., Wang, Z., Sun, Y., Guan, Q., Kang, L., Zhang, Q., Fang, L., Li, J., & Wong, J.
An acetylation-enhanced interaction between transcription factor Sox2 and the steroid receptor coactivators facilitates Sox2 transcriptional activity and function
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Co-IP
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Citation
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Deng, T., Hu, B., Wang, X., Lin, L., Zhou, J., Xu, Y., Yan, Y., Zheng, X., & Zhou, J.
Inhibition of Antiviral Innate Immunity by Avibirnavirus VP3 via Blocking TBK1-TRAF3 Complex Formation and IRF3 Activation. mSystems, 6(3), e00016-21.
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WB
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Human
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Citation
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Jiemin Wong,et al
An H3K14 ubiquitination-dependent SUV39H recruitment and activation is uniquely required for mammalian pericentromeric heterochromatin formation
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WB
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Human
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Citation
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Jiyong Zhou
Circovirus Transport Proceeds via Direct Interaction of the Cytoplasmic Dynein IC1 Subunit with theViral Capsid Protein
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WB
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IP
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Circovirus
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Citation
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Wang Shengnan,et al
Characterization and functional analysis of chicken promyelocytic leukemia protein
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WB
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Human
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Citation
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Xu Y, Qu M, He Y, et al
Smurf1 polyubiquitinates on K285/K282 of the kinases Mst1/2 to attenuate their tumor-suppressor functions
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WB
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IF
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Human
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Mouse
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Citation
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Min Liao
Rabies virus phosphoprotein P5 binding to BECN1 regulates self-replication by BECN1-mediated autophagy signaling pathway. Cell communication and signaling : CCS, 18(1), 153.
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WB
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CoIP
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human
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Citation
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Wang, X., Lin, L., Yu, Y., Yan, Y., Ojha, N. K., & Zhou, J.
The N-terminal residual arginine19 of influenza A virus NS1 protein is required for its nuclear localization and RNA binding. Veterinary microbiology, 251, 108895.
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Zhou, J., Qiu, Y., Zhu, N., Zhou, L., Dai, B., Feng, X., Hou, L., & Liu, J.
The Nucleolar Localization Signal of Porcine Circovirus Type 4 Capsid Protein Is Essential for Interaction With Serine-48 Residue of Nucleolar Phosphoprotein Nucleophosmin-1
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IP
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Citation
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Huang, W., Zhao, X., Ji, N., Guo, J., Feng, J., Chen, K., Wu, Y., Wang, J., Feng, H., & Zou, J.
IRF2 Cooperates with Phosphoprotein of Spring Viremia of Carp Virus to Suppress Antiviral Response in Zebrafish
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WB
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Human
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Citation
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Yi Luan ,et al
Decoupling actin assembly from microtubule disassembly by TBC1D3C-mediated direct GEF-H1 activation
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WB
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Human
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Citation
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Zhou, J., Li, J., Li, H., Zhang, Y., Dong, W., Jin, Y., Yan, Y., Gu, J., & Zhou, J.
The serine-48 residue of nucleolar phosphoprotein nucleophosmin-1 plays critical role in subcellular localization and interaction with porcine circovirus type 3 capsid protein. Veterinary research, 52(1), 4.
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WB
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Citation
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Wang X, Jiang X, Li B, et al
A regulatory circuit comprising the CBP and SIRT7 regulates FAM134B-mediated ER-phagy
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IP
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Citation
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Zhou, J., Wang, Y., Zhou, L., Qiu, Y., Zhao, J., Dai, B., Feng, X., Hou, L., & Liu, J.
Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
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WB
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Human
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Citation
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Yue Xiong,Qun-Ying Lei,Kun-Liang Guan
PARD3 induces TAZ activation and cell growth by promoting LATS1 and PP1 interaction
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WB
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human
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Citation
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Gao, L., Lu, Y., Chen, H. N., Li, Z., Hu, M., Zhang, R., Wang, X., Xu, Z., Gong, Y., Wang, R., Du, D., Hai, S., Li, S., Su, D., Li, Y., Xu, H., Zhou, Z. G., & Dai, L.
Deciphering the Clinical Significance and Kinase Functions of GSK3α in Colon Cancer by Proteomics and Phosphoproteomics
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WB
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Human
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Citation
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Jiyong Zhou,Liping Yan
Insights into species-specific regulation of ANP32A on the mammalianrestricted influenza virus polymerase activity
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WB
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CO-IP
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293T cells
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Citation
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-
TRIM21 restricts influenza A virus replication by ubiquitination-dependent degradation of M1
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WB
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Human
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Citation
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Zhou, J., Qiu, Y., Zhao, J., Wang, Y., Zhu, N., Wang, D., Cui, Y., Guo, J., Sun, T., Ji, Y., Wu, Z., Zeng, P., Li, J., Feng, X., Hou, L., & Liu, J.
The Network of Interactions between the Porcine Epidemic Diarrhea Virus Nucleocapsid and Host Cellular Proteins
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WB
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Pig
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Citation
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-
Wang, J., Liu, X. F., Zhang, H. Q., Allan, A. C., Wang, W. Q., & Yin, X. R.
Transcriptional and Post-transcriptional Regulation of Ethylene Biosynthesis by Exogenous Acetylsalicylic Acid in Kiwifruit
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WB
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Kiwifruit
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Citation
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The expanded ISG12 family in zebrafish: ISG12. 1 suppresses virus replication via targeting viral phosphoprotein
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ICC
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Fish
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Citation
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Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB
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WB
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Human
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Citation
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Di Wang
Bile Acids Control Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome
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WB
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human
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Citation
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Ojha, N. K., Liu, J., Yu, T., Fang, C., Zhou, J., & Liao, M.
Interplay of the ubiquitin proteasome system and the innate immune response is essential for the replication of infectious bronchitis virus. Archives of virology, 1–13. Advance online publication.
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WB
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Citation
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Tao P. Zhong
The Gridlock transcriptional repressor impedes vertebrate heart regeneration by restricting expression of lysine methyltransferase
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WB
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CoIP
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zebrafish
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Citation
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Wei Liu
TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction
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WB
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HEK293 cells
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Citation
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Gong-you Chen
Identification of 17 HrpX-Regulated Proteins Including Two Novel Type III Effectors,XOC_3956 and XOC_1550,in Xanthomonas oryzae pv. oryzicola
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WB
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Xanthomonas oryzae pv. oryzicola
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Citation
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Song Jiang,et al
Bipartite nuclear localization sequence is indispensable for nuclear import and stability of self-dimerization of ADARa in Bombyx mori
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WB
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Bombyx Mori
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Citation
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Zebrafish SETD3 mediated ubiquitination of phosphoprotein limits spring viremia of carp virus infection.
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Zebrafish
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Citation
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Wei, X., Zheng, Z., Feng, Z., Zheng, L., Tao, S., Zheng, B., Huang, B., Zhang, X., Liu, J., Chen, Y., Zong, W., Shan, Z., Fan, S., Chen, J., & Zhao, F.
Sigma-1 receptor attenuates osteoclastogenesis by promoting ER-associated degradation of SERCA2
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WB
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Human
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Citation
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Li Wen,et al
Mass Spectrometry-Based Proteomic Analysis of Potential Host Proteins Interacting with GP5 in PRRSV-Infected PAMs
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WB
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IP
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Pig
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Citation
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Mingliang Zhang;Dan Ye
The Zscan4-Tet2 Transcription Nexus RegulatesMetabolic Rewiring and Enhances Proteostasis toPromote Reprogramming
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IP
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WB
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human
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Citation
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Deng, T., Hu, B., Wang, X., Ding, S., Lin, L., Yan, Y., Peng, X., Zheng, X., Liao, M., Jin, Y., Dong, W., Gu, J., & Zhou, J.
TRAF6 autophagic degradation by avibirnavirus VP3 inhibits antiviral innate immunity via blocking NFKB/NF-κB activation
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WB
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Chicken
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Citation
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