NCAM1 Recombinant Mouse Monoclonal Antibody [PDH0-07] (HA601126)
Catalog# HA601126
Mouse Monoclonal to NCAM1
Tested for
-
IHC-P
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of NCAM1 on different lysates with Mouse anti-NCAM1 antibody (HA601126) at 1/2,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: Human brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 95 kDa
Observed band size: 140-250 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601126) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: Human brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 95 kDa
Observed band size: 140-250 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601126) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.