L1CAM Rabbit Polyclonal Antibody (0805-5)
Catalog# 0805-5
Rabbit Polyclonal to L1CAM
Tested for
-
WB
-
IF-Cell
-
IHC-P
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of L1CAM on different lysates with Rabbit anti-L1CAM antibody (0805-5) at 1/1,000 dilution.
Lane 1: A375 cell lysate
Lane 2: A549 cell lysate (low expression)
Lane 3: HeLa cell lysate
Lane 4: Human brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 140 kDa
Observed band size: 250 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0805-5) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A375 cell lysate
Lane 2: A549 cell lysate (low expression)
Lane 3: HeLa cell lysate
Lane 4: Human brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 140 kDa
Observed band size: 250 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0805-5) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.