Nkx2.2 Recombinant Rabbit Monoclonal Antibody [JE60-66] (HA722185)
Catalog# HA722185
Rabbit Monoclonal to Nkx2.2
Tested for
-
WB
-
IF-Cell
-
IHC-P
-
IF-Tissue
-
IHC-Fr
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Immunofluorescence analysis of frozen mouse E14.5 embyro tissue with Rabbit anti-Nkx2.2 antibody (HA722185) at 1/500 dilution.
Important Notice: Antigen retrieval is not required before IHC-Fr staining.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722185, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Important Notice: Antigen retrieval is not required before IHC-Fr staining.
The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722185, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).