NQO1 Mouse Monoclonal Antibody [13A1] (EM1801-21)
Catalog# EM1801-21
Mouse Monoclonal to NQO1
Tested for
-
WB
Reacts with
-
Human
-
Rat
Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (EM1801-21) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: SiHa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: Saos-2 cell lysate (low expression)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: SiHa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: Saos-2 cell lysate (low expression)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.