Nrf2 Recombinant Rabbit Monoclonal Antibody [JE30-14] (HA721432)
Catalog# HA721432
Rabbit Monoclonal to Nrf2
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WB
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IF-Cell
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Human
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Mouse
Western blot analysis of Nrf2 on different lysates with Rabbit anti-Nrf2 antibody (HA721432) at 1/1,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 2μM MG-132 for 18 hours whole cell lysate
Lane 3: HCT 116 whole cell lysate
Lane 4: HCT 116 treated with 25μM MG-132 for 4 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721432) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Nrf2 Recombinant Rabbit Monoclonal Antibody [JE30-14] (HA721432)
Immunogen
Synthetic peptide within human nrf2 aa 550-605.
Host
Rabbit
Positive Control
HeLa whole cell lysate, HeLa treated with 2μM MG-132 for 18 hours whole cell lysate, HCT 116 whole cell lysate, HCT 116 treated with 25μM MG-132 for 4 hours whole cell lysate, NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate, HCT 116.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE30-14
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 68 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Nrf2
SYNONYMS
erythroid derived 2 antibody;HEBP1 antibody;like 2 antibody;NF E2 related factor 2 antibody;NF-E2-related factor 2 antibody;NF2L2_HUMAN antibody;NFE2 related factor 2 antibody;NFE2-related factor 2 antibody;Nfe2l2 antibody;Nrf 2 antibody;NRF2 antibody;Nuclear factor (erythroid derived 2) like 2 antibody;Nuclear factor antibody;nuclear factor erythroid 2 like 2 antibody;Nuclear factor erythroid 2 related factor 2 antibody;Nuclear factor erythroid 2-related factor 2 antibody;Nuclear factor erythroid derived 2 like 2 antibody
SEQUENCE SIMILARITIES
Belongs to the bZIP family. CNC subfamily.
TISSUE SPECIFICITY
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated in the cytoplasm by the BCR(KEAP1) E3 ubiquitin ligase complex leading to its degradation. In response to oxidative stress, electrophile metabolites, such as sulforaphane, modify KEAP1, leading to inhibit activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and activity. In response to autophagy, the BCR(KEAP1) complex is inactivated (By similarity).; Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.; Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence.; Glycation impairs transcription factor activity by preventing heterodimerization with small Maf proteins. Deglycation by FN3K restores activity.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Nuclear factor erythroid 2-related factor 2 (NRF2), also known as nuclear factor erythroid-derived 2-like 2, is a transcription factor that in humans is encoded by the NFE2L2 gene. NRF2 is a basic leucine zipper (bZIP) protein that may regulate the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation, according to preliminary research.[6] In vitro, NRF2 binds to antioxidant response elements (AREs) in the promoter regions of genes encoding cytoprotective proteins. NRF2 induces the expression of heme oxygenase 1 in vitro leading to an increase in phase II enzymes. NRF2 also inhibits the NLRP3 inflammasome. NRF2 appears to participate in a complex regulatory network and performs a pleiotropic role in the regulation of metabolism, inflammation, autophagy, proteostasis, mitochondrial physiology, and immune responses.
CITATIONS
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Wu Peiyao,et al
Periodontal pathogen Fusobacterium nucleatum infection accelerates hepatic steatosis in high‐fat diet‐fed ApoE knockout mice by inhibiting Nrf2/Keap1 signaling
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WB
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Mouse
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Citation
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Taoyang Yuan,et al
Functional Polyphenol-Based Nanoparticles Boosted the Neuroprotective Effect of Riluzole for Acute Spinal Cord Injury
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IF
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Human
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Citation
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Ge Yuying,et al
Augmented Cornus officinalis Levels in Liuwei Dihuang Decoction Inhibits Nucleus Pulposus Cell Pyroptosis to Enhance Therapeutic Efficacy Against Intervertebral Disc Degeneration
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IF
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Mouse
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Citation
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Silver nanoparticles induced oxidative stress and mitochondrial injuries mediated autophagy in HC11 cells through Akt/AMPK/mTOR pathway
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WB
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Mouse
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Citation
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Activation of Nrf2 signaling by 4-octyl itaconate attenuates the cartilaginous endplate degeneration by inhibiting E3 ubiquitin ligase ZNF598
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WB
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IF-Cell
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Human
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Rat
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Citation
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Liu Y, Lin M, Mu X, et al
Protective effect of solanesol in glucose-induced hepatocyte injury: Mechanistic insights on oxidative stress and mitochondrial preservation
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WB
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Human
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Citation
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Identification and in silico analysis of novel antioxidant peptides in broken rice protein hydrolysate and its cytoprotective effect against H2O2-induced 2BS cell model
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WB
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Human
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Citation
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Melatonin attenuates lipopolysaccharide-induced immune dysfunction in dendritic cells
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WB
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Mouse
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Citation
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