NSUN4 Mouse Monoclonal Antibody [4G3] (HA600024)
Catalog# HA600024
Mouse Monoclonal to NSUN4
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
Western blot analysis of NSUN4 on different lysates with Mouse anti-NSUN4 antibody (HA600024) at 1/500 dilution.
Lane 1: MCF7 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: K-562 cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600024) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: K-562 cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600024) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.