Nucleolin Rabbit Polyclonal Antibody (R1307-3)
Catalog# R1307-3
Rabbit Polyclonal to Nucleolin
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
Western blot analysis of Nucleolin on different lysates with Rabbit anti-Nucleolin antibody (R1307-3) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: A431 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 100 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: A431 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 100 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
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