PAI1 Mouse Monoclonal Antibody [A9G8] (HA601152)
Catalog# HA601152
Mouse Monoclonal to PAI1
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WB
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IHC-P
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IF-Tissue
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FC
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Human
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Mouse
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Rat
Lane 1: no heat NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: no heat NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane)
Lane 5: HUVEC cell lysate (20 µg/Lane)
Lane 6: Mouse placenta tissue lysate (40 µg/Lane)
Lane 7: Rat placenta tissue lysate (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 45 kDa
Observed band size: 45/100 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601152) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (NBI02H) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
PAI1 Mouse Monoclonal Antibody [A9G8] (HA601152)
Immunogen
Synthetic peptide within human pai1 aa 31-80 / 402.
Host
Mouse
Positive Control
No heat NIH/3T3 cell lysate, no heat NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate, HUVEC cell lysate, mouse placenta tissue lysate, rat placenta tissue lysate, human breast carcinoma tissue, human placenta tissue, HepG2, NIH/3T3.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A9G8
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:5,000
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IF-Tissue
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1:1,000
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FC
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 45 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
PAI1
SYNONYMS
Clade E antibody;Endothelial plasminogen activator inhibitor antibody;Nexin antibody;Nexin plasminogen activator inhibitor type 1 antibody;PAI 1 antibody;PAI antibody;PAI-1 antibody;PAI1_HUMAN antibody;PLANH1 antibody;Plasminogen activator inhibitor 1 antibody;Plasminogen activator inhibitor type 1 antibody;Serine (or cysteine) proteinase inhibitor antibody;Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody;Serine proteinase inhibitor clade E member 1 antibody;serpin antibody;Serpin E1 antibody;Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody;Serpin peptidase inhibitor clade E antibody;Serpine 1 antibody;SERPINE1 antibody
SUBCELLULAR LOCATION
Secreted.
FUNCTION
Plasminogen activator inhibitor-1 (PAI-1) also known as endothelial plasminogen activator inhibitor or serpin E1 is a protein that in humans is encoded by the SERPINE1 gene. Elevated PAI-1 is a risk factor for thrombosis and atherosclerosis. PAI-1 is a serine protease inhibitor (serpin) that functions as the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). The other PAI, plasminogen activator inhibitor-2 (PAI-2) is secreted by the placenta and only present in significant amounts during pregnancy. In addition, protease nexin acts as an inhibitor of tPA and urokinase. PAI-1, however, is the main inhibitor of the plasminogen activators. PAI-1's main function entails the inhibition of urokinase plasminogen activator (uPA), an enzyme responsible for the cleavage of plasminogen to form plasmin. Plasmin mediates the degradation of the extracellular matrix either by itself or in conjunction with matrix metalloproteinases. In this scenario, PAI-1 inhibits uPA via active site binding, preventing the formation of plasmin. Additional inhibition is mediated by PAI-1 binding to the uPA/uPA receptor complex, resulting in the latter's degradation. Thus, PAI can be said to inhibit the serine proteases tPA and uPA/urokinase, and hence is an inhibitor of fibrinolysis, the physiological process that degrades blood clots. In addition, PAI-1 inhibits the activity of matrix metalloproteinases, which play a crucial role in invasion of malignant cells through the basal lamina. PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue.
CITATIONS
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Yuanyuan Liu,et al
Multi-omics and experimental analysis unveil the key components in Scutellaria baicalensis Georgi to alleviate hepatic fibrosis via regulating cPLA2-mediated arachidonic acid metabolism
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WB
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Mouse
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Citation
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