Patched / PTCH1 Recombinant Rabbit Monoclonal Antibody [PSH03-53] (HA722012)
Catalog# HA722012
Rabbit Monoclonal to Patched / PTCH1
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Patched / PTCH1 on different lysates with Rabbit anti-Patched / PTCH1 antibody (HA722012) at 1/10,000 dilution.
Lane 1: 293T-NT cell lysate
Lane 2: 293T-OE-PTCH1 cell lysate
Lysates/proteins at 1 µg/Lane.
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722012) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T-NT cell lysate
Lane 2: 293T-OE-PTCH1 cell lysate
Lysates/proteins at 1 µg/Lane.
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722012) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.