PGP9.5 Recombinant Rabbit Monoclonal Antibody [JM10-59] (ET1703-22)
Catalog# ET1703-22
Rabbit Monoclonal to PGP9.5
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
IP
-
FC
-
IHC-Fr
-
Human
-
Mouse
-
Rat
-
Cynomolgus monkey
-
Pig
Western blot analysis of PGP9.5 on different lysates with Rabbit anti-PGP9.5 antibody (ET1703-22) at 1/2,000 dilution.
Lane 1: A-172 cell lysate (20 µg/Lane)
Lane 2: SHG-44 cell lysate (20 µg/Lane)
Lane 3: U-87 MG cell lysate (20 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: NCI-H1299 cell lysate (20 µg/Lane)
Lane 6: A549 cell lysate (20 µg/Lane)
Lane 7: 293T cell lysate (20 µg/Lane)
Lane 8: Neuro-2a cell lysate (20 µg/Lane)
Lane 9: C6 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse brain tissue lysate (30 µg/Lane)
Lane 12: Rat brain tissue lysate (30 µg/Lane)
Lane 13: LNCaP cell lysate (negative) (20 µg/Lane)
Lane 14: K-562 cell lysate (negative) (20 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 7 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-22) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PGP9.5 Recombinant Rabbit Monoclonal Antibody [JM10-59] (ET1703-22)
Immunogen
Synthetic peptide within human pgp95 aa 191-223 / 223.
Host
Rabbit
Positive Control
A-172 cell lysate, SHG-44 cell lysate, U-87 MG cell lysate, SH-SY5Y cell lysate, NCI-H1299 cell lysate, A549 cell lysate, 293T cell lysate, Neuro-2a cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y, N2A, PC-12, mouse spinal cord tissue, mouse skin tissue, mouse brain tissue, mouse colon tissue, mouse cerebrum tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM10-59
RRID
APPLICATION DILUTION
-
WB
-
1:2,000-1:5,000
-
IF-Cell
-
1:100-1:500
-
IF-Tissue
-
1:100-1:1,000
-
IHC-P
-
1:1,000-1:5,000
-
IP
-
Use at an assay dependent concentration.
-
FC
-
1:1,000
-
IHC-Fr
-
1:200-1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 25 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PGP9.5
SYNONYMS
Epididymis luminal protein 117 antibody;Epididymis secretory protein Li 53 antibody;HEL 117 antibody;HEL S 53 antibody;NDGOA antibody;Neuron cytoplasmic protein 9.5 antibody;OTTHUMP00000218137 antibody;OTTHUMP00000218139 antibody;OTTHUMP00000218140 antibody;OTTHUMP00000218141 antibody;Park 5 antibody;PARK5 antibody;PGP 9.5 antibody;PGP9.5 antibody;PGP95 antibody;Protein gene product 9.5 antibody;Ubiquitin C terminal esterase L1 antibody;Ubiquitin C terminal hydrolase antibody;Ubiquitin C terminal hydrolase L1 antibody;Ubiquitin carboxyl terminal esterase L1 antibody;Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody;Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody;Ubiquitin thioesterase L1 antibody;Ubiquitin thiolesterase antibody;Ubiquitin thiolesterase L1 antibody;UCH-L1 antibody;UCHL1 antibody;UCHL1_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C12 family.
TISSUE SPECIFICITY
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
POST-TRANSLATIONAL MODIFICATION
O-glycosylated.
SUBCELLULAR LOCATION
Cytoplasm, Endoplasmic reticulum membrane.
FUNCTION
UCH-L1 (ubiquitin C-terminal hydrolase) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. UCH-L1 is expressed in brain neurons. Examination of specific brain regions reveals expression in all areas tested, particularly in the substantia nigra. UCH-L1 represents 1-2% of total soluble brain protein. Its occurrence in Lewy bodies and its function in the proteasome pathway make it a compelling candidate gene in Parkinson disease. The gene which encodes UCH-L1 maps to human chromosome 4p13. The 230 amino acid human UCH-L3 protein is 54% identical to that of UCH-L1. UCH-L3 is the predominant thiol protease and has high-affinity binding sites for ubiquitin.
CITATIONS
-
Xin Zikai,et al
Neuroprotective Effect of a Multistrain Probiotic Mixture in SOD1G93A Mice by Reducing SOD1 Aggregation and Targeting the Microbiota-Gut-Brain Axis
-
IF
-
Mouse
-
Citation
-
-
Xin Zikai,et al
Neuroprotective effect of a multi strain probiotic mixture in SOD1 G93A mice Through reducing SOD1 aggregation and targeting the microbe-gut-brain axis
-
IF
-
Mouse
-
Citation
-
-
Gastrodin protects porcine sertoli cells from zearalenone-induced abnormal secretion of glial cell line-derived neurotrophic factor through the NOTCH signaling pathway.
-
WB
-
Pig
-
Citation
-
