Phospho-Acetyl Coenzyme A Carboxylase (S79) Recombinant Rabbit Monoclonal Antibody [JE63-95] (HA721714)
Catalog# HA721714
Rabbit Monoclonal to Phospho-Acetyl Coenzyme A Carboxylase (S79)
Tested for
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WB
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-ACC (S79) on different lysates with Rabbit anti-Phospho-ACC (S79) antibody (HA721714) at 1/2,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 0.5μM Oligomycin for 30 minutes cell lysate
Lane 3: NIH/3T3 treated with 0.5μM Oligomycin for 30 minutes then treated with λpp for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 265 kDa
Observed band size: 265 kDa
Exposure time: 1 minutes 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721714) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 0.5μM Oligomycin for 30 minutes cell lysate
Lane 3: NIH/3T3 treated with 0.5μM Oligomycin for 30 minutes then treated with λpp for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 265 kDa
Observed band size: 265 kDa
Exposure time: 1 minutes 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721714) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.