Phospho-AKT (S473) Recombinant Rabbit Monoclonal Antibody [PSH04-44] (HA722129)
Catalog# HA722129
Rabbit Monoclonal to Phospho-AKT (S473)
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: MCF7 cell lysate
Lane 2: MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate
Lane 5: HEK-293 cell lysate
Lane 6: HEK-293 treated with 50μM LY294002 for 6 hours cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: NIH/3T3 treated with 100ng/mL PDGF for 5 minutes cell lysate
Lane 9: C6 cell lysate
Lane 10: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate
Lane 11: MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% BSA for 1 hour at room temperature. The primary antibody (HA722129) at 1/2,000 dilution and pan AKT antibody (HA721870) at 1/2,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-AKT (S473) Recombinant Rabbit Monoclonal Antibody [PSH04-44] (HA722129)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser473 of human akt1.
Specificity
Phospho-akt (ser473) [psh04-44] recombinant rabbit mab detects endogenous levels of akt1 only when phosphorylated at ser473. this antibody also recognizes akt2 and akt3 when phosphorylated at the corresponding residues.
Host
Rabbit
Modification
Phospho
Modification Site
S473
Positive Control
MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate, SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate, HEK-293 cell lysate, NIH/3T3 treated with 100ng/mL PDGF for 5 minutes cell lysate, C6 treated with 100ng/mL PDGF for 5 minutes cell lysate, mouse spleen tissue, rat spleen tissue, human breast cancer tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH04-44
APPLICATION DILUTION
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WB
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1:2,000-1:5,000
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IHC-P
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1:200
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FC
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1:10,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 56 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-AKT (S473)
SYNONYMS
AKT1 antibody;AKT1_HUMAN antibody;MGC99656 antibody;PKB antibody;PKB-ALPHA antibody;PRKBA antibody;Protein Kinase B Alpha antibody;Protein kinase B antibody;Proto-oncogene c-Akt antibody;RAC Alpha antibody;RAC antibody;RAC-alpha serine/threonine-protein kinase antibody;RAC-PK-alpha antibody;akt s473 antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Cell membrane.
FUNCTION
RAC(Rho family)-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) protein domains. It is commonly referred to as PKB, or by both names as "Akt/PKB". The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Phospho-Akt (Ser473) [PSH04-44] Recombinant Rabbit mAb detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues.
CITATIONS
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Li Zong,et al
Schisandrin A alleviates non-alcoholic fatty liver disease by improving liver metabolic disorders and suppressing the GSK3β signaling pathway
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WB
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Mouse
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Citation
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