Phospho-AMPK alpha 1 (S496) Recombinant Rabbit Monoclonal Antibody [SD0810] (ET1612-72)

UNIPROT #

Q13131

PROTEIN NAME

Phospho-AMPK alpha 1 (S496)

SYNONYMS

5 AMP activated protein kinase alpha 1catalytic subunit antibody;5 AMP activated protein kinase catalytic alpha 1 chain antibody;5' AMP activated protein kinase catalytic subunit alpha 1 antibody;5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody;AAPK1 antibody;AAPK1_HUMAN antibody;ACACA kinase antibody;acetyl CoA carboxylase kinase antibody;AI194361 antibody;AI450832 antibody;AL024255 antibody;AMP -activate kinase alpha 1 subunit antibody;AMP-activated protein kinase, catalytic, alpha -1 antibody;AMPK 1 antibody;AMPK alpha 1 antibody;AMPK alpha 1 chain antibody;AMPK antibody;AMPK subunit alpha-1 antibody;AMPK1 antibody;AMPKa1 antibody;AMPKalpha1 antibody;C130083N04Rik antibody;cb116 antibody;EC 2.7.11.1 antibody;HMG CoA reductase kinase antibody;HMGCR kinase antibody;hormone sensitive lipase kinase antibody;Hydroxymethylglutaryl CoA reductase kinase antibody;im:7154392 antibody;kinase AMPK alpha1 antibody;MGC33776 antibody;MGC57364 antibody;OTTHUMP00000161795 antibody;OTTHUMP00000161796 antibody;PRKAA 1 antibody;PRKAA1 antibody;Protein kinase AMP activated alpha 1 catalytic subunit antibody;SNF1-like protein AMPK antibody;SNF1A antibody;Tau protein kinase PRKAA1 antibody;wu:fa94c10 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated.; Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at a much lower level. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. Dephosphorylated by PPM1A and PPM1B.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

AMPK (5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. AMPKα1 (5'-AMP-activated protein kinase catalytic subunit alpha-1), also known as PRKAA1, is a 559 amino acid protein that belongs to the CAMK Ser/Thr protein kinase family and protein kinase superfamily. Highly phosphorylated, AMPKα1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 5p13.1.