Phospho-ATM (S1981) Recombinant Rabbit Monoclonal Antibody [JM93-25] (ET1705-50)
Catalog# ET1705-50
Rabbit Monoclonal to Phospho-ATM (S1981)
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
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Mouse
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 1μM Camptothecin for 1 hour cell lysate
Lane 3: HeLa treated with 1μM Camptothecin for 1 hour cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 351 kDa
Observed band size: 351 kDa
Exposure time: 3 minutes; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-ATM (S1981) Recombinant Rabbit Monoclonal Antibody [JM93-25] (ET1705-50)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser1981 of human atm aa 1,940-1,989 / 3,056.
Host
Rabbit
Modification
Phospho
Modification Site
S1981
Positive Control
HeLa cell lysate, HeLa treated with 1μM Camptothecin for 1 hour cell lysate, HeLa treated with 20μM Etoposide for 2 hours cell lysate, HepG2 cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, HeLa cells treated with 1μM Camptothecin for 1 hour, human breast carcinoma tissue, human colon cancer tissue, HEK-293.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM93-25
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1,000
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IP
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Use at an assay dependent concentration.
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 351 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-ATM (S1981)
SYNONYMS
A-T mutated antibody;A-T mutated homolog antibody;AT mutated antibody;AT1 antibody;ATA antibody;Ataxia telangiectasia mutated antibody;Ataxia telangiectasia mutated gene antibody;Ataxia telangiectasia mutated homolog (human) antibody;Ataxia telangiectasia mutated homolog antibody;ATC antibody;ATD antibody;ATDC antibody;ATE antibody;ATM antibody;ATM serine/threonine kinase antibody;ATM_HUMAN antibody;DKFZp781A0353 antibody;MGC74674 antibody;OTTHUMP00000232981 antibody;Serine protein kinase ATM antibody;Serine-protein kinase ATM antibody;Serine/threonine-protein kinase ATM antibody;Tefu antibody;TEL1 antibody;TEL1, telomere maintenance 1, homolog antibody;TELO1 antibody;Telomere fusion protein antibody
SEQUENCE SIMILARITIES
Belongs to the PI3/PI4-kinase family. ATM subfamily.
TISSUE SPECIFICITY
Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by NUAK1/ARK5. Autophosphorylation on Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-mediated activation of the kinase. During the late stages of DNA damage response, dephosphorylated following deacetylation by SIRT7, leading to ATM deactivation.; Acetylation, on DNA damage, is required for activation of the kinase activity, dimer-monomer transition, and subsequent autophosphorylation on Ser-1981. Acetylated in vitro by KAT5/TIP60. Deacetylated by SIRT7 during the late stages of DNA damage response, promoting ATM dephosphorylation and subsequent deactivation.
SUBCELLULAR LOCATION
Nucleus. Cytoplasmic vesicle.
FUNCTION
ATM serine/threonine kinase, symbol ATM, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Several of these targets, including p53, CHK2, BRCA1, NBS1 and H2AX are tumor suppressors. The ATM-mediated DNA damage response consists of a rapid and a delayed response. The protein kinase ATM may also be involved in mitochondrial homeostasis, as a regulator of mitochondrial autophagy (mitophagy) whereby old, dysfunctional mitochondria are removed. Increased ATM activity also occurs in viral infection where ATM is activated early during dengue virus infection as part of autophagy induction and ER stress response.
CITATIONS
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Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian Cancer
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WB
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human
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Citation
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TPP1 inhibits DNA damage response and chemosensitivity in esophageal cancer
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WB
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Human
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Citation
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Yang Liuqing,et al
Exploring the Anti-Ovarian Aging Mechanism of He's Yangchao Formula: Insights from Multi-Omics Analysis in Naturally Aged Mice
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WB
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Mouse
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Citation
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Xia, Z., Li, M., Hu, M., Lin, Y., Atteh, L. L., Fu, W., Gao, L., Bai, M., Huang, C., Yue, P., Liu, Y., & Meng, W.
Phosphoproteomics reveals that cinobufotalin promotes intrahepatic cholangiocarcinoma cell apoptosis by activating the ATM/CHK2/p53 signaling pathway
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WB
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Human
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Citation
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Wang, M., Xu, H., Li, T., Li, K., Zhang, Q., Chen, S., Zhao, L., Chen, J., & Chen, X.
Sonodynamic therapy of glioblastoma mediated by platelets with ultrasound-triggered drug release
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WB
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IHC
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Human
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Citation
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Fu, H., Liu, R., Jia, Z., Li, R., Zhu, F., Zhu, W., Shao, Y., Jin, Y., Xue, Y., Huang, J., Luo, K., Gao, X., Lu, H., & Zhou, Q.
Poly(ADP-ribosylation) of P-TEFb by PARP1 disrupts phase separation to inhibit global transcription after DNA damage
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WB
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Human
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Citation
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A novel and potent dihydroorotate dehydrogenase inhibitor suppresses the proliferation of colorectal cancer by inducing mitochondrial dysfunction and DNA damage
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WB
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Human
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Citation
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Wang Quanxin,et al
Taraxasterol enhanced bladder cancer cells radiosensitivity via inhibiting the COX-2/PGE2/JAK2/STAT3/MMP pathway
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WB
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Mouse
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Citation
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Disulfiram enhances the antitumor activity of cisplatin by inhibiting the Fanconi anemia repair pathway
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WB
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Human
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Citation
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