Phospho-Bcl-2 (S70) Recombinant Rabbit Monoclonal Antibody [PSH11-02] (HA723269)
Catalog# HA723269
Rabbit Monoclonal to Phospho-Bcl-2 (S70)
Tested for
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WB
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IF-Cell
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FC
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-Bcl-2 (S70) on different lysates with Rabbit anti-Phospho-Bcl-2 (S70) antibody (HA723269) at 1/2,000 dilution.
Lane 1: Jurrkat cell lysate
Lane 2: Jurrkat treated with 1μM paclitaxel overnight cell lysate
Lane 3: Jurrkat treated with 1μM paclitaxel overnight cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 1 minute 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723269) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurrkat cell lysate
Lane 2: Jurrkat treated with 1μM paclitaxel overnight cell lysate
Lane 3: Jurrkat treated with 1μM paclitaxel overnight cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 1 minute 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723269) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.