Phospho-Beta Catenin (T41/S45) Recombinant Rabbit Monoclonal Antibody [JE54-02] (HA722316)

UNIPROT #

P35222

PROTEIN NAME

Phospho-Beta Catenin (T41/S45)

SYNONYMS

b-catenin antibody;Beta catenin antibody;Beta-catenin antibody;Cadherin associated protein antibody;Catenin (cadherin associated protein), beta 1, 88kDa antibody;Catenin beta 1 antibody;Catenin beta-1 antibody;CATNB antibody;CHBCAT antibody;CTNB1_HUMAN antibody;CTNNB antibody;CTNNB1 antibody;DKFZp686D02253 antibody;FLJ25606 antibody;FLJ37923 antibody;OTTHUMP00000162082 antibody;OTTHUMP00000165222 antibody;OTTHUMP00000165223 antibody;OTTHUMP00000209288 antibody;OTTHUMP00000209289 antibody

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Cell membrane.

FUNCTION

The cellular level of β-catenin is mostly controlled by its ubiquitination and proteosomal degradation. The E3 ubiquitin ligase TrCP1 (also known as β-TrCP) can recognize β-catenin as its substrate through a short linear motif on the disordered N-terminus. However, this motif (Asp-Ser-Gly-Ile-His-Ser) of β-catenin needs to be phosphorylated on the two serines in order to be capable to bind β-TrCP. Phosphorylation of the motif is performed by Glycogen Synthase Kinase 3 alpha and beta (GSK3α and GSK3β). GSK3s are constitutively active enzymes implicated in several important regulatory processes. There is one requirement, though: substrates of GSK3 need to be pre-phosphorylated four amino acids downstream (C-terminally) of the actual target site. Thus it also requires a "priming kinase" for its activities. In the case of β-catenin, the most important priming kinase is Casein Kinase I (CKI). Once a serine-threonine rich substrate has been "primed", GSK3 can "walk" across it from C-terminal to N-terminal direction, phosphorylating every 4th serine or threonine residues in a row. This process will result in dual phosphorylation of the aforementioned β-TrCP recognition motif as well.