Phospho-Cofilin (S3) Recombinant Rabbit Monoclonal Antibody [PSH07-18] (HA722806)
Catalog# HA722806
Rabbit Monoclonal to Phospho-Cofilin (S3)
Tested for
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WB
Reacts with
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Human
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Rat
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Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-Cofilin (S3) on different lysates with Rabbit anti-Phospho-Cofilin (S3) antibody (HA722806) at 1/2,000 dilution.
Lane 1: COS-1 cell lysate
Lane 2: C6 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: MCF7 cell lysate
Lane 5: A431 cell lysate
Lane 6: A431 cell lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722806) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: COS-1 cell lysate
Lane 2: C6 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: MCF7 cell lysate
Lane 5: A431 cell lysate
Lane 6: A431 cell lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722806) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.