Phospho-JNK1/2/3 (T183 + T183 + T221) Recombinant Rabbit Monoclonal Antibody [ST500] (ET1609-42)
Catalog# ET1609-42
Rabbit Monoclonal to Phospho-JNK1/2/3 (JNK1(T183)+JNK2(T183)+JNK3(T221))
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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IHC-Fr
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Human
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Mouse
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Rat
Lane 1: Hela cell lysate, untreated
Lane 2: Hela cell lysate, treated with Anisomycin
Lane 3: A431 cell lysate, untreated
Lane 4: A431 cell lysate, treated with UV40
Lane 5: 293 cell lysate, untreated
Lane 6: 293 cell lysate, treated with UV40
Lane 7: Hela cell lysate, untreated
Lane 8: Hela cell lysate, treated with UV40
Lysates/proteins at 10 µg/Lane.
Predicted band size: 48/53 kDa
Observed band size: 48/53 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-42) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-JNK1/2/3 (T183 + T183 + T221) Recombinant Rabbit Monoclonal Antibody [ST500] (ET1609-42)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding thr183 of human jnk1 aa 161-204 / 427.
Host
Rabbit
Modification
Phospho
Modification Site
JNK1(T183)+JNK2(T183)+JNK3(T221)
Positive Control
NIH/3T3 cell lysate treated with Anisomycin, Hela cell lysate treated with Anisomycin, A431 cell lysate treated with UV40, 293 cell lysate treated with UV40, Hela cell lysate treated with UV40, Hela, NIH/3T3, HUVEC, human colon tissue, human endometrium tissue, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
ST500
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:1,000
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
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IHC-Fr:1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 48/53 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-JNK1/2/3 (JNK1(T183)+JNK2(T183)+JNK3(T221))
SYNONYMS
C Jun kinase 2 antibody;c Jun N terminal kinase 1 antibody;c Jun N terminal kinase 2 antibody;c Jun N terminal kinase 3 antibody;c-Jun N-terminal kinase 1 antibody;JNK 46 antibody;JNK 55 antibody;JNK antibody;JNK-46 antibody;JNK1 antibody;JNK1A2 antibody;JNK2 antibody;JNK21B1/2 antibody;JNK2A antibody;JNK2ALPHA antibody;JNK2B antibody;JNK2BETA antibody;JNK3 alpha protein kinase antibody;JNK3 antibody;JNK3A antibody;Jun kinase antibody;JUN N terminal kinase antibody;MAP kinase 10 antibody;MAP kinase 8 antibody;MAP kinase 9 antibody;MAP kinase p49 3F12 antibody;MAPK 10 antibody;MAPK 8 antibody;MAPK 9 antibody;MAPK10 antibody;mapk8 antibody;MAPK9 antibody;Mitogen activated protein kinase 10 antibody;Mitogen activated protein kinase 8 antibody;Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody;Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody;Mitogen activated protein kinase 9 antibody;Mitogen-activated protein kinase 8 antibody;MK08_HUMAN antibody;p493F12 antibody;p54a antibody;p54aSAPK antibody;p54bSAPK antibody;PRKM10 antibody;PRKM8 antibody;PRKM9 antibody;SAPK antibody;SAPK(beta) antibody;SAPK1 antibody;SAPK1a antibody;SAPK1b antibody;SAPK1c antibody;Stress activated protein kinase 1 antibody;Stress activated protein kinase 1a antibody;Stress activated protein kinase 1b antibody;Stress activated protein kinase 1c antibody;Stress activated protein kinase beta antibody;Stress activated protein kinase JNK1 antibody;Stress activated protein kinase JNK2 antibody;Stress activated protein kinase JNK3 antibody;Stress-activated protein kinase 1c antibody;Stress-activated protein kinase JNK1 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
POST-TRANSLATIONAL MODIFICATION
Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2. May be phosphorylated at Thr-183 and Tyr-185 by MAP3K1/MEKK1. Phosphorylated form is more concentrated at synapses than none-phosphorylated (By similarity).
SUBCELLULAR LOCATION
Cytoplasm, Membrane, Mitochondrion, Nucleus.
FUNCTION
JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.
CITATIONS
-
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-
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-
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-
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-
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-
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-
-
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-
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-
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-
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-
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-
-
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-
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-
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-
-
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-
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-
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-
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-
-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
-
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-
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-
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-
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-
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-
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-
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-
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-
-
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-
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-
-
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-
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-
-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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