Phospho-MDM2 (S166) Recombinant Rabbit Monoclonal Antibody [JE64-20] (HA721791)

UNIPROT #

Q00987

PROTEIN NAME

Phospho-MDM2 (S166)

SYNONYMS

ACTFS antibody;Double minute 2 protein antibody;E3 ubiquitin-protein ligase Mdm2 antibody;Hdm 2 antibody;Hdm2 antibody;HDMX antibody;MDM 2 antibody;MDM2 antibody;MDM2 oncogene E3 ubiquitin protein ligase antibody;Mdm2 p53 E3 ubiquitin protein ligase homolog antibody;Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody;MDM2_HUMAN antibody;MDM2BP antibody;Mouse Double Minute 2 antibody;MTBP antibody;Murine Double Minute Chromosome 2 antibody;Oncoprotein Mdm2 antibody;p53 Binding Protein Mdm2 antibody;p53-binding protein Mdm2 antibody;Ubiquitin protein ligase E3 Mdm2 antibody;Ubiquitin protein ligase E3 Mdm2 antibody

SEQUENCE SIMILARITIES

Belongs to the MDM2/MDM4 family.

TISSUE SPECIFICITY

Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on Ser-166 by SGK1 activates ubiquitination of p53/TP53. Phosphorylated at multiple sites near the RING domain by ATM upon DNA damage; this prevents oligomerization and E3 ligase processivity and impedes constitutive p53/TP53 degradation.; Autoubiquitination leads to proteasomal degradation; resulting in p53/TP53 activation it may be regulated by SFN. Also ubiquitinated by TRIM13. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitination and degradation of p53/TP53. Deubiquitinated by USP7 leading to its stabilization.

SUBCELLULAR LOCATION

Nucleus, nucleoplasm, nucleolus, cytoplasm.

FUNCTION

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.