Phospho-MEK1/2 (S218/S222) Recombinant Rabbit Monoclonal Antibody [ST0490] (ET1609-50)
Catalog# ET1609-50
Rabbit Monoclonal to Phospho-MEK1/2 (S218/S222)
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate
Lane 3: C6 cell lysate
Lane 4: C6 treated with 200nM PMA for 30 minutes cell lysate
Lane 5: NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lane 6: C6 treated with 200nM PMA for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-MEK1/2 (S218/S222) Recombinant Rabbit Monoclonal Antibody [ST0490] (ET1609-50)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser218 and 222 of human mek1 aa 200-249 / 393.
Host
Rabbit
Modification
Phospho
Modification Site
S218/S222
Positive Control
NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, C6 treated with 200nM PMA for 30 minutes cell lysate, Hela cell lysate, A431 cell lysate, 293T cell lysate, NIH/3T3 cells treated with 200nM PMA for 30 minutes, Hela, HepG2, human tonsil tissue, human liver carcinoma tissue, human spleen tissue, human breast carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
ST0490
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 43 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-MEK1/2 (S218/S222)
SYNONYMS
Dual specificity mitogen activated protein kinase kinase 1 antibody;Dual specificity mitogen-activated protein kinase kinase 1 antibody;ERK activator kinase 1 antibody;MAP kinase kinase 1 antibody;MAP kinase/Erk kinase 1 antibody;MAP2K1 antibody;MAPK/ERK kinase 1 antibody;MAPKK 1 antibody;MAPKK1 antibody;MEK 1 antibody;Mek1 antibody;MEKK1 antibody;Mitogen activated protein kinase kinase 1 antibody;MKK 1 antibody;MKK1 antibody;MP2K1_HUMAN antibody;PRKMK1 antibody;Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody;Protein kinase mitogen activated, kinase 1 antibody;hide
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
TISSUE SPECIFICITY
Widely expressed, with extremely low levels in brain.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation.; Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
SUBCELLULAR LOCATION
Cytoplasm, Cytoskeleton, Membrane, Nucleus.
FUNCTION
Activation of extracellular signal-regulated kinase (ERK) or mitogen-activated protein kinase by MEK (mitogen-activated protein kinase or extracellular signal-regulated kinase kinase) is an essential event in the mitogenic growth factor-induced signal transduction pathway. Phosphorylation of MEKs correlates with their ability to phosphorylate and activate ERKs. MEK1 and MEK2 can also be activated by autophosphorylation. Lipopolysaccharide activates many of the MAPK family members of the immediate upstream MAPK activator MEK1, MEK2, and MEK3. In plants, MEK can phosphorylate and activate MAPK, and that Tyr phosphorylation is critical for the catalytic activity of MAPK in plants.
CITATIONS
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Liu Yakun,et al
A rare germline BMP15 missense mutation causes hereditary ovarian immature teratoma in human
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IHC
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Mouse
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Citation
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Downregulation of a CT10 regulator of kinase (Crk) promotes the formation of diapause embryos in the brine shrimp Artemia
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WB
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Brine shrimp Artemia
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Citation
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Chen Jiahe,et al
ETS1 and RBPJ transcriptionally regulate METTL14 to suppress TGF-β1-induced epithelial-mesenchymal transition in human bronchial epithelial cells
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WB
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Human
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Mouse
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Citation
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He, Y., Wang, X., Lu, W., Zhang, D., Huang, L., Luo, Y., Xiong, L., Li, H., Zhang, P., Li, Q., & Liang, S.
PGK1 contributes to tumorigenesis and sorafenib resistance of renal clear cell carcinoma via activating CXCR4/ERK signaling pathway and accelerating glycolysis
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WB
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Human
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Citation
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