Phospho-Rb (S807) Recombinant Rabbit Monoclonal Antibody [SR3-82] (ET1602-36)

UNIPROT #

P06400

PROTEIN NAME

Phospho-Rb (S807)

SYNONYMS

OSRC antibody;Osteosarcoma antibody;p105-Rb antibody;P105RB antibody;PP105 antibody;pp110 antibody;PPP1R130 antibody;pRb antibody;Prepro retinoblastoma associated protein antibody;Protein phosphatase 1 regulatory subunit 130 antibody;Rb antibody;RB_HUMAN antibody;RB1 antibody;RB1 gene antibody;Retinoblastoma 1 antibody;Retinoblastoma suspectibility protein antibody;Retinoblastoma-associated protein antibody

SEQUENCE SIMILARITIES

Belongs to the retinoblastoma protein (RB) family.

TISSUE SPECIFICITY

Expressed in the retina. Expressed in foreskin keratinocytes (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis (By similarity).; N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.; Acetylated during keratinocyte differentiation. Acetylation at Lys-873 and Lys-874 regulates subcellular localization. Can be deacetylated by SIRT1.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Pediatric cancer retinoblastoma and the formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene (Rb). The Rb protein regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle at G1-S with transcriptional machinery. During G1, cyclin D-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser-807/ Ser 811 and Thr 821/Thr 826. Hypophosphorylated Rb represses the transcription of genes controlling the cell cycle through direct protein-protein interactions and through the recruitment of histone deacetylase.