Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1] (ET1612-32)
Catalog# ET1612-32
Rabbit Monoclonal to Phospho-Smad2 (S250)
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WB
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FC
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IP
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 200nM PMA for 30 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-32) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1] (ET1612-32)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser250 of human smad2 aa 221-270 / 467.
Host
Rabbit
Modification
Phospho
Modification Site
S250
Positive Control
HeLa cell lysate, HeLa treated with 200nM PMA for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 200nM PMA for 30 minutes cell lysate, C6 cell lysate, C6 treated with 200nM PMA for 30 minutes cell lysate, HeLa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD207-1
RRID
APPLICATION DILUTION
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WB
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1:5,000
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FC
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1:50-1:100
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 52 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Smad2 (S250)
SYNONYMS
Drosophila, homolog of, MADR2 antibody;hMAD-2 antibody;HsMAD2 antibody;JV18 antibody;JV18-1 antibody;JV181 antibody;MAD antibody;MAD homolog 2 antibody;MAD Related Protein 2 antibody;Mad-related protein 2 antibody;MADH2 antibody;MADR2 antibody;MGC22139 antibody;MGC34440 antibody;Mother against DPP homolog 2 antibody;Mothers against decapentaplegic homolog 2 antibody;Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody;Mothers against DPP homolog 2 antibody;OTTHUMP00000163489 antibody;Sma and Mad related protein 2 antibody;Sma- and Mad-related protein 2 MAD antibody;SMAD 2 antibody;SMAD family member 2 antibody;SMAD, mothers against DPP homolog 2 antibody;SMAD2 antibody;SMAD2_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the dwarfin/SMAD family.
TISSUE SPECIFICITY
Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. TGF-beta-induced Ser-465/467 phosphorylation declines progressively in a KMT5A-dependent manner. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. Phosphorylated by PDPK1.; In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation. Monoubiquitinated, leading to prevent DNA-binding (By similarity). Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Ubiquitinated by RNF111, leading to its degradation: only SMAD2 proteins that are 'in use' are targeted by RNF111, RNF111 playing a key role in activating SMAD2 and regulating its turnover (By similarity).; Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
CITATIONS
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CD44 mediates hyaluronan to promote the differentiation of human amniotic mesenchymal stem cells into chondrocytes
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WB
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Human
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Citation
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Ouyang, Z. Q., Shao, L. S., Wang, W. P., Ke, T. F., Chen, D., Zheng, G. R., Duan, X. R., Chu, J. X., Zhu, Y., Yang, L., Shan, H. Y., Huang, L., & Liao, C. D.
Low intensity pulsed ultrasound ameliorates Adriamycin-induced chronic renal injury by inhibiting ferroptosis
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WB
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Rat
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Citation
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Xue, F., Zhao, Z., Gu, Y., Han, J., Ye, K., & Zhang, Y.
7,8-Dihydroxyflavone modulates bone formation and resorption and ameliorates ovariectomy-induced osteoporosis. eLife, 10, e64872.
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WB
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Rat
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Citation
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Wu Bibo,et al
PD-1 Inhibitor Aggravate Irradiation-Induced Myocardial Fibrosis by Regulating TGF-β1/Smads Signaling Pathway via GSDMD-Mediated Pyroptosis
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WB
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Mouse
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Citation
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TBX20 Contributes to Balancing the Differentiation of Perivascular Adipose-Derived Stem Cells to Vascular Lineages and Neointimal Hyperplasia
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WB
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Mouse
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Citation
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Plasmodium manipulates the expression of host long non-coding RNA during red blood cell intracellular infection
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WB
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Citation
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CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ
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WB
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Mouse
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Citation
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Jiang Shuanghong,et al
ARHGAP4 promotes colon cancer metastasis through the TGF-β signaling pathway and may be associated with T cell exhaustion
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WB
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Human
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Citation
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