Phospho-STAT1 (Y701) Recombinant Rabbit Monoclonal Antibody [PSH04-02] (HA722083)
Catalog# HA722083
Rabbit Monoclonal to Phospho-STAT1 (Y701)
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WB
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IF-Cell
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Human
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL IFNγ for 30 minutes cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa treated with 50ng/mL IFNα1 for 30 minutes cell lysate
Lane 5: A431 cell lysate
Lane 6: A431 treated with 100ng/mL EGF for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 91/84 kDa (STAT1α/β)
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722083) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-STAT1 (Y701) Recombinant Rabbit Monoclonal Antibody [PSH04-02] (HA722083)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding tyr701 of human stat1.
Host
Rabbit
Modification
Phospho
Modification Site
Y701
Positive Control
HeLa treated with 100ng/mL IFNγ for 30 minutes cell lysate, HeLa treated with 50ng/mL IFNα1 for 30 minutes cell lysate, A431 treated with 100ng/mL EGF for 30 minutes cell lysate, HeLa cells treated with 100ng/mL IFNα1 for 5 minutes.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH04-02
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 87 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-STAT1 (Y701)
SYNONYMS
Signal transducer and activator of transcription 1 91kD antibody;CANDF7 antibody;DKFZp686B04100 antibody;IMD31A antibody;IMD31B antibody;IMD31C antibody;ISGF 3 antibody;ISGF-3 antibody;OTTHUMP00000163552 antibody;OTTHUMP00000165046 antibody;OTTHUMP00000165047 antibody;OTTHUMP00000205845 antibody;Signal transducer and activator of transcription 1 91kDa antibody;Signal transducer and activator of transcription 1 antibody;Signal transducer and activator of transcription 1, 91kD antibody;Signal transducer and activator of transcription 1-alpha/beta antibody;STAT 1 antibody;Stat1 antibody;STAT1_HUMAN antibody;STAT91 antibody;Transcription factor ISGF 3 components p91 p84 antibody;Transcription factor ISGF-3 components p91/p84 antibody;Transcription factor ISGF3 components p91/p84 antibody;XStat1 antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Signal transducer and activator of transcription 1 (STAT1) is a transcription factor which in humans is encoded by the STAT1 gene. It is a member of the STAT protein family. All STAT molecules are phosphorylated by receptor associated kinases, that causes activation, dimerization by forming homo- or heterodimers and finally translocate to nucleus to work as transcription factors. Specifically STAT1 can be activated by several ligands such as Interferon alpha (IFNα), Interferon gamma (IFNγ), Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Interleukin 6 (IL-6), or IL-27.
CITATIONS
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Ming-Ming Zheng,et al
IMPDH inhibitors upregulate PD-L1 in cancer cells without impairing immune checkpoint inhibitor efficacy
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WB
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Mouse
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Citation
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