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PI 3 Kinase p85 alpha Recombinant Rabbit Monoclonal Antibody [SU04-07] (ET1608-70)

Price: Regular price $385.00

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Catalog# ET1608-70

Rabbit Monoclonal to PI 3 Kinase p85 alpha

Tested for

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

PI 3 Kinase p85 alpha Recombinant Rabbit Monoclonal Antibody [SU04-07] (ET1608-70)

Immunogen

Synthetic peptide within c-terminal human pi 3 kinase p85 alpha.

Host

Rabbit

Positive Control

MCF-7 cell lysate, Raji cell lysate, Hela, MCF-7, HepG2, NIH/3T3, human liver carcinoma tissue, human kidney tissue, mouse kidney tissue, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU04-07

RRID

AB_3069823

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:1,000

  • FC

  • 1:50-1:100

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 84 kDa

Isotype

IgG

TARGET

UNIPROT #

P27986

PROTEIN NAME

PI 3 Kinase p85 alpha

SYNONYMS

GRB1 antibody;p85 alpha antibody;p85 antibody;P85A_HUMAN antibody;Phosphatidylinositol 3 kinase associated p 85 alpha antibody;Phosphatidylinositol 3 kinase regulatory 1 antibody;Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody;Phosphatidylinositol 3-kinase regulatory subunit alpha antibody;Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody;PI3 kinase p85 subunit alpha antibody;PI3-kinase regulatory subunit alpha antibody;PI3-kinase subunit p85-alpha antibody;PI3K antibody;PI3K regulatory subunit alpha antibody;Pik3r1 antibody;PtdIns 3 kinase p85 alpha antibody;PtdIns-3-kinase regulatory subunit alpha antibody;PtdIns-3-kinase regulatory subunit p85-alpha antibody

SEQUENCE SIMILARITIES

Belongs to the PI3K p85 subunit family.

TISSUE SPECIFICITY

Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

POST-TRANSLATIONAL MODIFICATION

Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.; Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.

SUBCELLULAR LOCATION

Cytoplasm, membrane, nucleus.

FUNCTION

Phosphatidylinositol 3-kinase (PI 3-kinase) phosphorylates the 3' OH position of the inositol ring of inositol lipids and is composed of p85 and p110 subunits. PI 3-kinase p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. PI 3-kinase p85α, also known as GRB1, phosphatidylinositol 3-kinase regulatory 1 or p85, is a 724 amino acid protein that exists as four alternatively spliced isoforms. Involved in insulin metabolism, defects in the PI 3-kinase p85α gene have been linked to insulin resistance. PI 3-kinase p85α is polyubiquitinated in T-cells by Cbl-b, and has multiple phosphorylated amino acid residues, including a phosphorylated tyrosine residue at position 467.

CITATIONS

  • Liu Xinying,et al

    Genipin promotes the apoptosis and autophagy of neuroblastoma cells by suppressing the PI3K/AKT/mTOR pathway

  • Jian Li; Caiqiao Zhang

    Metformin Prevents Follicular Atresia in Aging Laying Chickens through Activation of PI3K/AKT and Calcium Signaling Pathways

  • Qiu Huanhuan,et al

    Sr-Incorporated Bioactive Glass Remodels the Immunological Microenvironment by Enhancing the Mitochondrial Function of Macrophage via the PI3K/AKT/mTOR Signaling Pathway

  • Li Xin,et al

    Exploring the Osteogenic Effects of Simiao Wan through Activation of the PI3K/AKT Pathway in Osteoblasts

  • Hu Jiahe,et al

    Engineered-Doping Strategy for Self-Sufficient Reactive Oxygen Species Blossom to Amplify Ferroptosis/Cuproptosis Sensibilization in Hepatocellular Carcinoma Treatment

  • Zhou Peijie,et al

    Ferulic Acid Methyl Ester Attenuates Cerebral Ischemia-Reperfusion Injury in Rats by Modulating PI3K/HIF-1α/VEGF Signaling Pathway

  • Yao Peixue,et al

    IL-17A regulates collagen synthesis after autophagy in bronchial fibroblasts through the PI3K/AKT/mTOR pathway

  • Xu, F., Wu, J., Hu, Y., Chu, C., Liu, W., Li, X., Zheng, W., Yang, W., Zhao, B., Guo, J., Wang, Z., Jia, Y., & Xiao, W.

    Mechanisms of action underlying the effect of Tongsaimai on wound healing based on experimental and network pharmacology

  • Chun Li ,et al

    Ruminococcus bromii-generated acetate alleviated Clonorchis sinensis-induced liver fibrosis in mice

  • Wanshun Wang,et al

    Microenvironment-responsive injectable hydrogel for neuro-vascularized bone regeneration

  • Yirong Wu, Siyao Zhan, Lian Chen, Mingrui Sun, Miaofu Li, Xuanting Mou, Zhen Zhang, Linhao Xu, Yizhou Xu

    TNFSF14/LIGHT promotes cardiac fibrosis and atrial fibrillation vulnerability via PI3Kγ/SGK1 pathway-dependent M2 macrophage polarisation

  • Jiang Huangyu,et al

    Evodiamine Inhibits the Progression of Esophageal Aquamous Cell Carcinoma via Modulating PI3K/AKT/mTOR Pathway

  • Tian Xiaoling,et al

    The Therapeutic Potential of Chi Shao and Fu Zi in Acute-on-Chronic Liver Failure: Insights from Network Pharmacology and Animal Studies

  • Wen, S., Yang, K., Bai, Y., Wu, Y., Liu, D., Wu, X., Zhang, X., & Sun, J.

    Investigating the Mechanism of Action of Schisandra chinensis Combined with Coenzyme Q10 in the Treatment of Heart Failure Based on PI3K-AKT Pathway

  • Rao Zichen

    HNRNP I promotes IRAK1 degradation to reduce podocyte apoptosis and inflammatory response alleviating renal injury in diabetic nephropathy

  • Xin H, Tang Y, Jin YH, et al

    Knockdown of LMNA inhibits Akt/β-catenin-mediated cell invasion and migration in clear cell renal cell carcinoma cells

  • Li, J., Zhang, X., Guo, D., Shi, Y., Zhang, S., Yang, R., & Cheng, J.

    The mechanism of action of paeoniae radix rubra-angelicae sinensis radix drug pair in the treatment of rheumatoid arthritis through PI3K/AKT/NF-κB signaling pathway

  • Na Lu,et al

    CORO1C Regulates the Malignant Biological Behavior of Ovarian Cancer Cells and Modulates the mRNA Expression Profile through the PI3K/AKT Signaling Pathway

  • Kuijun Zhan,et al

    Network pharmacology to unveil the mechanism of Astragali Radix in the treatment of lupus nephritis via PI3K/AKT/mTOR pathway

  • Shen Y

    The age-related changes and differences in energy metabolism and glutamate-glutamine recycling in the d-gal-induced and naturally occurring senescent astrocytes in vitro.

  • Minglu Zhou,et al

    Cell surface patching via CXCR4-targeted nanothreads for cancer metastasis inhibition

  • Lushu Chen, Yuan Cao, Yaming Shen, Huan Li, Rong Ye, Jin Yao

    Downregulation of PIK3IP1 in retinal microglia promotes retinal pathological neovascularization via PI3K-AKT pathway activation

  • Gao Yuelan,et al

    Inhibiting PRMT1 protects against CoNV by regulating macrophages through the FGF2/PI3K/Akt pathway

  • A network pharmacology approach and experimental validation to investigate the anticancer mechanism and potential active targets of ethanol extract of Wei-Tong-Xin against colorectal cancer through induction of apoptosis via PI3K/AKT signaling pathway

  • Carvedilol exhibits anti-acute T lymphoblastic leukemia effect in vitro and in vivo via inhibiting β-ARs signaling pathway

  • Nickel nanoparticles affect the migration and invasion of HTR-8/SVneo cells by downregulating MMP2 through the PI3K/AKT pathway

  • Luo, C., Zhou, M., Chen, C., Li, S., Li, Q., Huang, Y., & Zhou, Z.

    A liposome-based combination strategy using doxorubicin and a PI3K inhibitor efficiently inhibits pre-metastatic initiation by acting on both tumor cells and tumor-associated macrophages

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