PI3-kinase p110 subunit beta Recombinant Rabbit Monoclonal Antibody [SC0580] (ET1610-36)
Catalog# ET1610-36
Rabbit Monoclonal to PI3-kinase p110 subunit beta
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WB
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IP
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IHC-P
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FC
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Human
Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lane 3: HeLa cell lysate
Lane 4: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 123 kDa
Observed band size: 110 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PI3-kinase p110 subunit beta Recombinant Rabbit Monoclonal Antibody [SC0580] (ET1610-36)
Immunogen
Synthetic peptide within human pik3cb aa 5-52 / 1070.
Host
Rabbit
Positive Control
Jurkat cell lysate, K-562 cell lysate, HeLa cell lysate, MCF7 cell lysate, human liver carcinoma tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC0580
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:50-1:100
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 123 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PI3-kinase p110 subunit beta
SYNONYMS
5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody;5-bisphosphate 3-kinase catalytic subunit beta isoform antibody;DKFZp779K1237 antibody;MGC133043 antibody;OTTHUMP00000216901 antibody;OTTHUMP00000216904 antibody;p110 BETA antibody;p110Beta antibody;Phosphatidylinositol 3 kinase catalytic beta polypeptide antibody;Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta antibody;Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform antibody;Phosphatidylinositol-4 antibody;Phosphoinositide 3 kinase catalytic beta polypeptide antibody;PI3 kinase p110 subunit beta antibody;PI3-kinase subunit beta antibody;PI3K antibody;PI3K beta antibody;PI3K-beta antibody;PI3Kbeta antibody;PI3KCB antibody;PIK3C1 antibody;Pik3cb antibody;PK3CB_HUMAN antibody;PtdIns 3 kinase p110 antibody;PtdIns-3-kinase subunit beta antibody;PtdIns-3-kinase subunit p110-beta antibody
SEQUENCE SIMILARITIES
Belongs to the PI3/PI4-kinase family.
TISSUE SPECIFICITY
Expressed ubiquitously.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-1070 down-regulates lipid kinase activity.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110 expression is restricted to white blood cells. It has been shown to bind p85α and β, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and β γ heterotrimeric G proteins.
CITATIONS
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Hu Qin,et al
Capsaicin Attenuates LPS-Induced Acute Lung Injury by Inhibiting Inflammation and Autophagy Through Regulation of the TRPV1/AKT Pathway
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WB
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Human
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Citation
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Yue Xiao,et al
Dietary oxygenated water mitigates type 2 diabetes mellitus by modulating gut microbiota and enhancing glucose metabolism in skeletal muscle
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WB
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Rabbit
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Citation
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