PKM2 Rabbit Polyclonal Antibody (R1603-5)
Catalog# R1603-5
Rabbit Polyclonal to PKM2
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Western blot analysis of PKM2 on different lysates with Rabbit anti-PKM2 antibody (R1603-5) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate (20 µg/Lane)
Lane 6: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane)
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 13 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1603-5) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
PKM2 Rabbit Polyclonal Antibody (R1603-5)
Immunogen
Synthetic peptide within human pkm2 aa 381-430.
Host
Rabbit
Positive Control
HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HeLa, NIH/3T3, C6, human liver tissue, mouse liver tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:5,000
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IF-Cell
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1:200
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IHC-P
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1:10,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 58 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PKM2
SYNONYMS
CTHBP antibody;Cytosolic thyroid hormone binding protein antibody;Cytosolic thyroid hormone-binding protein antibody;KPYM_HUMAN antibody;MGC3932 antibody;OIP 3 antibody;OIP-3 antibody;OIP3 antibody;OPA interacting protein 3 antibody;Opa-interacting protein 3 antibody;p58 antibody;PK muscle type antibody;PK, muscle type antibody;PK2 antibody;PK3 antibody;PKM antibody;PKM2 antibody;pykm antibody;Pyruvate kinase 2/3 antibody;Pyruvate kinase 3 antibody;Pyruvate kinase isozymes M1/M2 antibody;Pyruvate kinase muscle antibody;Pyruvate kinase muscle isozyme antibody;pyruvate kinase PKM antibody;Pyruvate kinase, muscle 2 antibody;TCB antibody;THBP1 antibody;Thyroid hormone binding protein 1 antibody;Thyroid hormone binding protein cytosolic antibody;Thyroid hormone-binding protein 1 antibody;Tumor M2 PK antibody;Tumor M2-PK antibody
SEQUENCE SIMILARITIES
Belongs to the pyruvate kinase family.
TISSUE SPECIFICITY
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
POST-TRANSLATIONAL MODIFICATION
ISGylated.; Under hypoxia, hydroxylated by EGLN3.; Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.; FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus
FUNCTION
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival . Promotes in a STAT1-dependent manner, the expression of the immune checkpoint protein CD274 in ARNTL/BMAL1-deficient macrophages (By similarity).
CITATIONS
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Cui Aimin,et al
Low-level laser therapy alleviates periodontal age-related inflammation in diabetic mice via the GLUT1/mTOR pathway
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IF
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Mouse
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Citation
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Mu Mingshan,et al
FAM188B promotes the growth, metastasis, and invasion of hepatocellular carcinoma by targeting the HRNPA1/PKM2 axis
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WB
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Human
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Citation
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Nie Qian,et al
Integration RNA bulk and single cell RNA sequencing to explore the change of glycolysis-related immune microenvironment and construct prognostic signature in head and neck squamous cell carcinoma
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WB
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Human
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Citation
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