Plectin Recombinant Rabbit Monoclonal Antibody [SY29-04] (ET1607-80)
Catalog# ET1607-80
Rabbit Monoclonal to Plectin
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WB
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IHC-P
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IP
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IF-Tissue
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Plectin KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 532 kDa
Observed band size: 532 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-80) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Plectin Recombinant Rabbit Monoclonal Antibody [SY29-04] (ET1607-80)
Immunogen
Recombinant protein within human plectin aa 2-101 / 4,684.
Host
Rabbit
Positive Control
Human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, HeLa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY29-04
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:200-1:1,000
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IP
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1-2μg/sample
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IF-Tissue
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1:100-1:200
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 532 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Plectin
SYNONYMS
EBS1 antibody;EBSO antibody;HD1 antibody;Hemidesmosomal protein 1 antibody;PCN antibody;pleC antibody;PLEC_HUMAN antibody;PLEC1 antibody;PLEC1b antibody;Plectin 1 antibody;Plectin 1 intermediate filament binding protein 500kDa antibody;Plectin 6 antibody;Plectin antibody;Plectin-1 antibody;PLTN antibody
SEQUENCE SIMILARITIES
Belongs to the plakin or cytolinker family.
TISSUE SPECIFICITY
Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
SUBCELLULAR LOCATION
Cell junction, Cytoplasm, Cytoskeleton.
FUNCTION
Plectin is a giant protein found in nearly all mammalian cells which acts as a link between the three main components of the cytoskeleton: actin microfilaments, microtubules and intermediate filaments. Plectin links the cytoskeleton to junctions found in the plasma membrane that structurally connect different cells. By holding these different networks together, plectin plays an important role in maintaining the mechanical integrity and viscoelastic properties of tissues. Plectin binds both Lamin B and vimentin, and this binding is regulated by a variety of protein kinases. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions.
CITATIONS
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Gineste, C., Youhanna, S., Vorrink, S. U., Henriksson, S., Hernández, A., Cheng, A. J., Chaillou, T., Buttgereit, A., Schneidereit, D., Friedrich, O., Hultenby, K., Bruton, J. D., Ivarsson, N., Sandblad, L., Lauschke, V. M., & Westerblad, H.
Enzymatically dissociated muscle fibers display rapid dedifferentiation and impaired mitochondrial calcium control
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IF
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Mouse
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Citation
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