PPP2R5B Rabbit Polyclonal Antibody (HA500409)
Catalog# HA500409
Rabbit Polyclonal to PPP2R5B
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Human cerebellum tissue lysate
Lane 3: Rat cerebellum tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500409) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
PPP2R5B Rabbit Polyclonal Antibody (HA500409)
Immunogen
Recombinant protein within human ppp2r5b aa 1-250.
Host
Rabbit
Positive Control
Mouse cerebellum tissue lysate, human cerebellum tissue lysate, rat cerebellum tissue lysate, SH-SY5Y cell lysates, human colon tissue, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IHC-P
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1:600
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FC
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2.1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 57 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PPP2R5B
SYNONYMS
2A5B_HUMAN antibody;B56B antibody;Beta isoform of regulatory subunit B56 protein phosphatase 2A antibody;FLJ35411 antibody;PP2A B subunit B' beta isoform antibody;PP2A B subunit B56 beta isoform antibody;PP2A B subunit isoform B' beta antibody;PP2A B subunit isoform B''-beta antibody;PP2A B subunit isoform B56-beta antibody;PP2A B subunit isoform PR61-beta antibody;PP2A B subunit isoform R5-beta antibody;PP2A B subunit PR61 beta isoform antibody;PP2A B subunit R5 beta isoform antibody;PPP2R5B antibody;PR 61B antibody;PR61B antibody;Protein phosphatase 2 regulatory subunit B (B56) beta antibody;Protein phosphatase 2 regulatory subunit B (B56) beta isoform antibody;Protein phosphatase 2 regulatory subunit B' beta antibody;Protein phosphatase 2 regulatory subunit B' beta isoform antibody;Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit beta isoform antibody;Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform antibody
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform is an enzyme that in humans is encoded by the PPP2R5B gene. The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B56 subfamily. Highest expression in brain.
CITATIONS
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DNA damage repair-related gene signature for identifying the immune status and predicting the prognosis of hepatocellular carcinoma
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IHC
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Human
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Mouse
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Citation
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