PSMA Mouse Monoclonal Antibody [A9H9] (HA601164)
Catalog# HA601164
Mouse Monoclonal to PSMA
Tested for
-
WB
-
IHC-P
-
IF-Cell
Reacts with
-
Human
Western blot analysis of PSMA on different lysates with Mouse anti-PSMA antibody (HA601164) at 1/1,000 dilution.
Lane 1: LNCaP cell lysate
Lane 2: 22RV1 cell lysate
Lane 3: PC-3M cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 100/200 kDa
Exposure time: 2 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601164) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.
Lane 1: LNCaP cell lysate
Lane 2: 22RV1 cell lysate
Lane 3: PC-3M cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 100/200 kDa
Exposure time: 2 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601164) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.